Document Detail


Honokiol induces apoptosis via cytochrome c release and caspase activation in activated rat hepatic stellate cells in vitro.
MedLine Citation:
PMID:  15678380     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The therapeutic goal in liver fibrosis is the reversal of fibrosis and the selective clearance of activated hepatic stellate cells (HSCs) by inducing apoptosis. Over the past several years, we have screened for natural products that mediate apoptosis in activated HSCs. Among the candidate compounds, honokiol, isolated from Magnoliae cortex, was found to induce apoptotic death in activated rat HSCs, while there was no cell viability change in hepatocytes, at concentrations of 12.5-50 microM. Apoptosis was identified by DNA fragmentation, activation of caspase-3 and -9, and the proteolytic cleavage of poly(ADP-ribose) polymerase, down-regulation of bcl-2 and the release of mitochondrial cytochrome c into the cytoplasm.
Authors:
Eun-Jeon Park; Yu-Zhe Zhao; Young Ho Kim; Byung-Hoon Lee; Dong Hwan Sohn
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Planta medica     Volume:  71     ISSN:  0032-0943     ISO Abbreviation:  Planta Med.     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2005-01-28     Completed Date:  2005-03-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0066751     Medline TA:  Planta Med     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  82-4     Citation Subset:  IM    
Affiliation:
Department of Pharmacy, Medicinal Resources Research Center, Wonkwang University, Iksan, Jeonbuk, Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antineoplastic Agents, Phytogenic / administration & dosage,  pharmacology*,  therapeutic use
Apoptosis / drug effects*
Biphenyl Compounds / administration & dosage,  pharmacology*,  therapeutic use
Caspases / metabolism
Cytochromes c / metabolism
Dose-Response Relationship, Drug
Drugs, Chinese Herbal / administration & dosage,  pharmacology*,  therapeutic use
Enzyme Activation / drug effects
Flow Cytometry
Hepatocytes / drug effects,  enzymology,  metabolism
Lignans / administration & dosage,  pharmacology*,  therapeutic use
Magnolia*
Male
Phytotherapy*
Rats
Rats, Sprague-Dawley
Chemical
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 0/Biphenyl Compounds; 0/Drugs, Chinese Herbal; 0/Lignans; 35354-74-6/honokiol; 9007-43-6/Cytochromes c; EC 3.4.22.-/Caspases

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