Document Detail


Homology model for oncostatin M based on NMR structural data.
MedLine Citation:
PMID:  9692947     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Oncostatin M (OM) is a member of the cytokine family which regulates the proliferation and differentiation of a variety of cell types and includes interleukin-6 (IL-6), leukemia inhibitory factor (LIF), and granulocyte-colony stimulating factor (G-CSF). This family of proteins adopts a four-helix bundle fold with up-up-down-down topology and contains intramolecular disulfide bonds. Since an X-ray or NMR structure for OM is not currently available, a homology model for OM was determined from the X-ray structures of human growth hormone (hGH), LIF, and G-CSF where the alignment was based on secondary structure instead of sequence. The OM secondary structure was determined from NMR structural data, and the secondary structures for hGH, LIF, and G-CSF were obtained from the reported X-ray structures. The resulting homology model was refined using sequential NOE distance 13C restraints, chemical shift information, and a conformational database.
Authors:
D Kitchen; R C Hoffman; F J Moy; R Powers
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Biochemistry     Volume:  37     ISSN:  0006-2960     ISO Abbreviation:  Biochemistry     Publication Date:  1998 Jul 
Date Detail:
Created Date:  1998-08-21     Completed Date:  1998-08-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  10581-8     Citation Subset:  IM    
Affiliation:
Immunex Corporation, Seattle, Washington 98101, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Cattle
Crystallography, X-Ray
Cytokines / chemistry*
Granulocyte Colony-Stimulating Factor / chemistry
Growth Inhibitors / chemistry
Human Growth Hormone / chemistry
Humans
Interleukin-6*
Leukemia Inhibitory Factor
Lymphokines / chemistry
Models, Molecular*
Molecular Sequence Data
Nuclear Magnetic Resonance, Biomolecular
Oncostatin M
Peptides / chemistry*
Protein Structure, Secondary
Sequence Homology, Amino Acid*
Chemical
Reg. No./Substance:
0/Cytokines; 0/Growth Inhibitors; 0/Interleukin-6; 0/LIF protein, human; 0/Leukemia Inhibitory Factor; 0/Lymphokines; 0/OSM protein, human; 0/Peptides; 106956-32-5/Oncostatin M; 12629-01-5/Human Growth Hormone; 143011-72-7/Granulocyte Colony-Stimulating Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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