| Homology model for oncostatin M based on NMR structural data. | |
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MedLine Citation:
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PMID: 9692947 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Oncostatin M (OM) is a member of the cytokine family which regulates the proliferation and differentiation of a variety of cell types and includes interleukin-6 (IL-6), leukemia inhibitory factor (LIF), and granulocyte-colony stimulating factor (G-CSF). This family of proteins adopts a four-helix bundle fold with up-up-down-down topology and contains intramolecular disulfide bonds. Since an X-ray or NMR structure for OM is not currently available, a homology model for OM was determined from the X-ray structures of human growth hormone (hGH), LIF, and G-CSF where the alignment was based on secondary structure instead of sequence. The OM secondary structure was determined from NMR structural data, and the secondary structures for hGH, LIF, and G-CSF were obtained from the reported X-ray structures. The resulting homology model was refined using sequential NOE distance 13C restraints, chemical shift information, and a conformational database. |
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Authors:
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D Kitchen; R C Hoffman; F J Moy; R Powers |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Biochemistry Volume: 37 ISSN: 0006-2960 ISO Abbreviation: Biochemistry Publication Date: 1998 Jul |
Date Detail:
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Created Date: 1998-08-21 Completed Date: 1998-08-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370623 Medline TA: Biochemistry Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 10581-8 Citation Subset: IM |
Affiliation:
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Immunex Corporation, Seattle, Washington 98101, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Cattle Crystallography, X-Ray Cytokines / chemistry* Granulocyte Colony-Stimulating Factor / chemistry Growth Inhibitors / chemistry Human Growth Hormone / chemistry Humans Interleukin-6* Leukemia Inhibitory Factor Lymphokines / chemistry Models, Molecular* Molecular Sequence Data Nuclear Magnetic Resonance, Biomolecular Oncostatin M Peptides / chemistry* Protein Structure, Secondary Sequence Homology, Amino Acid* |
| Chemical | |
Reg. No./Substance:
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0/Cytokines; 0/Growth Inhibitors; 0/Interleukin-6; 0/LIF protein, human; 0/Leukemia Inhibitory Factor; 0/Lymphokines; 0/OSM protein, human; 0/Peptides; 106956-32-5/Oncostatin M; 12629-01-5/Human Growth Hormone; 143011-72-7/Granulocyte Colony-Stimulating Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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