Document Detail

Homology between legumin-like polypeptides from cereals and pea.
MedLine Citation:
PMID:  3985947     Owner:  NLM     Status:  MEDLINE    
The presence of legumin-like constituents within the globulin fractions of wheat (Triticum aestivum), rye (Secale cereale) and corn (maize, Zea mays) was demonstrated. Two-dimensional analysis of wheat globulins in the presence and absence of a reducing agent revealed the existence of reducible approximately 60 kDa polypeptides. Western-blot analysis with 125I-labelled antibodies raised against the oat (Avena sativa) 12S globulin holoprotein or its alpha-subunits demonstrated, firstly, the immunological homology between the alpha- and beta-subunits of pea (Pisum sativum) legumin and oat 12S globulin, and secondly, the similar occurrence in wheat of antigenically homologous approximately 20kDa and approximately 40 kDa polypeptides that associate via disulphide linkage to form approximately 60 kDa dimers. Western blotting also showed the presence of disulphide-linked approximately 20 kDa and approximately 40 kDa legumin-like subunits within the globulin fractions of rye and corn.
L S Robert; C Nozzolillo; I Altosaar
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Biochemical journal     Volume:  226     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1985 Mar 
Date Detail:
Created Date:  1985-05-23     Completed Date:  1985-05-23     Revised Date:  2010-09-10    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  847-52     Citation Subset:  IM    
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MeSH Terms
Cereals / analysis
Electrophoresis, Polyacrylamide Gel
Fabaceae / analysis
Globulins / analysis
Peptides / analysis*,  immunology
Plant Proteins*
Plants / analysis*
Plants, Medicinal
Species Specificity
Vegetable Proteins / analysis*
Reg. No./Substance:
0/Globulins; 0/Peptides; 0/Plant Proteins; 0/Vegetable Proteins; 0/legumin protein, plant

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