Document Detail


Homologous region 3 from Bombyx mori nucleopolyhedrovirus enhancing the transcriptional activity of heat shock cognate 70-4 promoter from Bombyx mori and Bombyx mandarina in vitro and in vivo.
MedLine Citation:
PMID:  15914923     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The heat shock cognate 70-4 protein gene promoter (HSC70-4p) from Bombyx mori (BmHSC70-4p) is an ideal candidate for the transgenic silkworm due to its high transcriptional activity, and the homologous region 3 from Bombyx mori nucleopolyhedrovirus (BmNPVhr3) functions as an enhancer for several promoters. Using luciferase as a reporter gene and transient expression system in vivo and in vitro, we found that BmNPVhr3 can significantly increase the transcriptional activity of BmHSC70-4p and HSC70-4p from Bombyx mandarina (BmandHSC70-4p). Moreover, the transcriptional activity of the combination of BmHSC70-4p and BmNPVhr3 changed with developmental stages and hormone titers. These results suggest that the combination of BmHSC70-4 promoter and BmNPVhr3 enhancer is more effective candidate for the transgene or stable cell expression system in Bombyx mori.
Authors:
Shunming Tang; Qiaoling Zhao; Yongzhu Yi; Zhifang Zhang; Yiren Li
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  69     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-05-25     Completed Date:  2005-09-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1014-7     Citation Subset:  IM    
Affiliation:
Key Laboratory of Silkworm Biotechnology, Ministry of Agriculture, Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bombyx / genetics*,  virology*
Cell Line
Gene Expression Regulation, Developmental
Heat-Shock Proteins / genetics*
Insect Proteins / genetics*
Juvenile Hormones / physiology
Larva
Nucleopolyhedrovirus / genetics*
Regulatory Sequences, Nucleic Acid
Time Factors
Transcription, Genetic*
Chemical
Reg. No./Substance:
0/Heat-Shock Proteins; 0/Insect Proteins; 0/Juvenile Hormones

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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