| Homogeneous luminescence decay time-based assay using energy transfer from nanospheres. | |
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MedLine Citation:
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PMID: 12033319 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Following a study on the feasibility of resonance energy transfer (RET) from carboxylated nanospheres with an incorporated phosphorescent donor to a cationic polyelectrolyte/acceptor aggregate on their surface, a novel scheme for homogeneous assays is presented that is based on RET from phosphorescent biotinylated nanospheres to fluorescently labeled streptavidin (SA). The phosphorescent nanospheres, with a diameter of well below 50 nm, are made from carboxylated polyacrylonitrile and dyed with ruthenium(II)-tris-4,7-diphenyl-1,10-phenanthroline dichloride (Ru(dpp)). Due to the small size of the nanospheres and the complete extraction of the ruthenium dye into the nanospheres during the precipitation process, RET occurs from Ru(dpp) to the label if labeled SA binds to the surface of the nanospheres. Luminescence quenching by oxygen or other species present in the sample can be neglected due to the shielding effect of the polymer matrix. Based on this finding, a competitive binding assay was established, where avidin and labeled SA compete for the biotin binding sites on the nanosphere. The process of binding to the surface can be detected by measurement of the luminescence intensity or the apparent decay time which is in the order of 2.5-4.5 micros. |
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Authors:
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Jens M Kümer; Otto S Wolfbeis; Ingo Klimant |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Analytical chemistry Volume: 74 ISSN: 0003-2700 ISO Abbreviation: Anal. Chem. Publication Date: 2002 May |
Date Detail:
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Created Date: 2002-05-29 Completed Date: 2003-04-04 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: United States |
Other Details:
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Languages: eng Pagination: 2151-6 Citation Subset: IM |
Affiliation:
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Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biotinylation Energy Transfer Feasibility Studies Half-Life Immunoassay / methods* Luminescent Measurements* Microspheres Nanotechnology / methods* Ruthenium Compounds |
| Chemical | |
Reg. No./Substance:
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0/Ruthenium Compounds |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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