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Homofermentative production of D: -lactic acid from sucrose by a metabolically engineered Escherichia coli.
MedLine Citation:
PMID:  22791225     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Escherichia coli W, a sucrose-positive strain, was engineered for the homofermentative production of D: -lactic acid through chromosomal deletion of the competing fermentative pathway genes (adhE, frdABCD, pta, pflB, aldA) and the repressor gene (cscR) of the sucrose operon, and metabolic evolution for improved anaerobic cell growth. The resulting strain, HBUT-D, efficiently fermented 100 g sucrose l(-1) into 85 g D: -lactic acid l(-1) in 72-84 h in mineral salts medium with a volumetric productivity of ~1 g l(-1) h(-1), a product yield of 85 % and D: -lactic acid optical purity of 98.3 %, and with a minor by-product of 4 g acetate l(-1). HBUT-D thus has great potential for production of D: -lactic acid using an inexpensive substrate, such as sugar cane and/or beet molasses, which are primarily composed of sucrose.
Authors:
Yongze Wang; Tian Tian; Jinfang Zhao; Jinhua Wang; Tao Yan; Liyuan Xu; Zao Liu; Erin Garza; Andrew Iverson; Ryan Manow; Chris Finan; Shengde Zhou
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-13
Journal Detail:
Title:  Biotechnology letters     Volume:  -     ISSN:  1573-6776     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Key Laboratory of Fermentation Engineering (Ministry of Education), College of Bioengineering, Hubei University of Technology, Wuhan, 430068, People's Republic of China, wyzzju@126.com.
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