Document Detail

Homocysteine and its thiolactone may promote apoptotic events in blood platelets in vitro.
MedLine Citation:
PMID:  20701459     Owner:  NLM     Status:  In-Process    
The actions of homocysteine and its major metabolite, cyclic thioester, homocysteine thiolactone on endothelial cells, blood platelets, plasmatic fibrinogen and plasminogen--the important major components of haemostasis, regulating the flowing properties of blood--are complex and sometimes controversial. Homocysteine (Hcys) can promote apoptosis in endothelial cells, but the role of Hcys and its thiolactone in the apoptotic process in blood platelets is unknown. In order to study the appearance of apoptosis in platelets after treatment with the reduced form of Hcys or its thiolactone different markers were chosen: annexin V binding (phosphatidylserine exposure), platelet microparticle formation, mitochondrial membrane depolarization and αIIbβ3 expression in vitro. Apoptotic events and platelet activation were measured by a flow cytometer. In gel-filtered platelets treated with different concentrations of the reduced form of Hcys (25, 50 and 100 µM, 10 min) a significant increase of phosphatidylserine exposure (about 37% at the highest concentration, p < 0.001) and platelet microparticle formation were observed. Homocysteine caused also a dose-dependent depolarization of mitochondrial potential. The same apoptotic markers appeared in HTL-treated platelets (0.2 and 1 µM). Moreover, resveratrol (25 µM), a well known antioxidant, distinctly reduced the level of apoptotic markers. The obtained results indicate that Hcys and its thiolactone may promote in vitro apoptotic events in human gel-filtered platelets.
Beata Olas; Joanna Malinowska; Joanna Rywaniak
Related Documents :
2810939 - A possible mechanism for the gastric mucosal protection by oren-gedoku-to (ogt), a trad...
22803109 - Effects and mechanisms of hemopoiesis-stimulating activity of immobilized oligonucleoti...
15448739 - Nitric oxide and platelet energy metabolism.
22876309 - Coagulation changes during presyncope and recovery.
22906019 - Increased platelet activation and thrombosis in transgenic mice expressing constitutive...
4089839 - Glutathione levels in human platelets display a circadian rhythm in vitro.
3298449 - The carboxylpropeptide of type i procollagen in skin fibrillogenesis.
25243089 - Von-willebrand disease presenting as intractable epistaxis after nasal polypectomy.
3663949 - Thrombin-induced changes in platelet membrane glycoproteins ib, ix, and iib-iiia complex.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Platelets     Volume:  21     ISSN:  1369-1635     ISO Abbreviation:  Platelets     Publication Date:  2010  
Date Detail:
Created Date:  2010-10-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9208117     Medline TA:  Platelets     Country:  England    
Other Details:
Languages:  eng     Pagination:  533-40     Citation Subset:  IM    
Department of General Biochemistry, Institute of Biochemistry, University of Łódź, Banacha 12/16, 90-237 Łódź, Poland.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Soluble CD40 ligand is elevated in type 1 diabetic nephropathy but not predictive of mortality, card...
Next Document:  Use of glycoprotein IIb/IIIa receptor antagonist in prosthetic valve thrombosis.