Document Detail

Homocysteine increases neuronal damage in hippocampal slices receiving oxygen and glucose deprivation.
MedLine Citation:
PMID:  17103328     Owner:  NLM     Status:  MEDLINE    
Homocystinuria is an inherited metabolic disorder caused by severe deficiency of cystationine beta-synthase activity, resulting in the tissue accumulation of homocysteine (Hcy). Affected patients usually present many signs and symptoms such as seizures, mental retardation, atherosclerosis and stroke. The aim of this study is to evaluate in vivo and in vitro effects of Hcy using hippocampal slices from Wistar rats exposed to oxygen and glucose deprivation (OGD), followed by reoxygenation, an in vitro model of hypoxic-ischemic events. Neural cell injury was quantified by the measurement of lactate dehydrogenase (LDH) released from damaged cells into the extracellular fluid. The results showed that both in vivo and in vitro Hcy increased the LDH released to de incubation medium, suggesting an increase of tissue damage caused by OGD. This fact can be related with the high incidence of stroke in homocystinuric patients.
Bárbara Tagliari; Lauren L Zamin; Christianne G Salbego; Carlos Alexandre Netto; Angela T S Wyse
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-11-14
Journal Detail:
Title:  Metabolic brain disease     Volume:  21     ISSN:  0885-7490     ISO Abbreviation:  Metab Brain Dis     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2006-12-15     Completed Date:  2007-02-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8610370     Medline TA:  Metab Brain Dis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  273-8     Citation Subset:  IM    
Departamento de Bioquímica, ICBS, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600-Anexo, CEP 90035-003, Porto Alegre, RS, Brazil.
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MeSH Terms
Brain Ischemia / metabolism*,  pathology
Cell Death
Glucose / pharmacology
Hippocampus / metabolism*,  pathology
Homocysteine / blood,  pharmacology*
Homocystinuria / metabolism*,  pathology
L-Lactate Dehydrogenase / metabolism
Nerve Degeneration / metabolism*,  pathology
Organ Culture Techniques
Oxygen / pharmacology
Rats, Wistar
Reperfusion Injury / metabolism,  pathology
Reg. No./Substance:
454-28-4/Homocysteine; 50-99-7/Glucose; 7782-44-7/Oxygen; EC Dehydrogenase

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