Document Detail

Homocysteine increases methionine synthase mRNA level in Caco-2 cells.
MedLine Citation:
PMID:  15319544     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Methionine synthase (MTR) synthesizes methionine from homocysteine, using cobalamin as a cofactor and 5-methyltetrahydrofolate as a cosubstrate. AIM: To determine the influence of homocystine (Hcy, dimer of homocysteine) in the presence of either cobalamin or methionine on the transcription and the activity of methionine synthase in Caco-2, a human adenocarcinoma cell line. METHODS: Methionine synthase activity and quantification of its mRNA by real-time RT-PCR were determined in cells cultivated under four differents conditions: Hcy with cobalamin (Hcy+ Cbl+), Hcy with methionine (Hcy+Met+), methionine with Cbl (Met+ Cbl+) and methionine only (Met+). RESULTS: Activity (nmol/h/mg protein) was maximal in cells cultivated in Hcy+Cbl+ (2.45 +/- 0.35), compared to cells cultivated in Hcy+Met+ (0.18 +/- 0.01, p<0.001), in Met+ Cbl+ (1.60 +/- 0.06, p<0.05), and in Met+ (0.40 +/- 0.05, p<0.001), suggesting an adaptation of the cells to requirement in synthesized methionine. The mRNA level of MTR in Hcy+ Cbl+ and Hcy+Met+ (2.82 +/-0.49 and 3.33 +/- 0.48 AU, respectively ) was about 2.5 / 3.0-fold higher than that in Met+ Cbl+ and in Met+ (1.00 +/-0.13 and 1.20 +/-0.20 AU, respectively, p<0.001). CONCLUSION: Methionine synthase expression of Caco-2 cell is under a transcriptional control influenced by Hcy.
Sandrine Ortiou; Jean-Marc Alberto; Jean-Louis Guéant; Marc Merten
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology     Volume:  14     ISSN:  1015-8987     ISO Abbreviation:  Cell. Physiol. Biochem.     Publication Date:  2004  
Date Detail:
Created Date:  2004-08-20     Completed Date:  2005-02-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9113221     Medline TA:  Cell Physiol Biochem     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  407-14     Citation Subset:  IM    
Laboratoire de Pathologie Cellulaire et Moléculaire en Nutrition, Equipe INSERM 0014, Faculté de Médecine, Vandoeuvre-les-Nancy, France.
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MeSH Terms
5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase / analysis,  biosynthesis,  genetics*
Adenocarcinoma / chemistry,  enzymology,  genetics*
Caco-2 Cells
Gene Expression Regulation, Neoplastic*
Homocysteine / pharmacology,  physiology*
Methionine / pharmacology,  physiology
RNA, Messenger / analysis,  metabolism
Transcription, Genetic / genetics
Vitamin B 12 / pharmacology,  physiology
Reg. No./Substance:
0/RNA, Messenger; 454-28-4/Homocysteine; 63-68-3/Methionine; 68-19-9/Vitamin B 12; EC S-Methyltransferase

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