| Homocysteine increases methionine synthase mRNA level in Caco-2 cells. | |
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MedLine Citation:
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PMID: 15319544 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Methionine synthase (MTR) synthesizes methionine from homocysteine, using cobalamin as a cofactor and 5-methyltetrahydrofolate as a cosubstrate. AIM: To determine the influence of homocystine (Hcy, dimer of homocysteine) in the presence of either cobalamin or methionine on the transcription and the activity of methionine synthase in Caco-2, a human adenocarcinoma cell line. METHODS: Methionine synthase activity and quantification of its mRNA by real-time RT-PCR were determined in cells cultivated under four differents conditions: Hcy with cobalamin (Hcy+ Cbl+), Hcy with methionine (Hcy+Met+), methionine with Cbl (Met+ Cbl+) and methionine only (Met+). RESULTS: Activity (nmol/h/mg protein) was maximal in cells cultivated in Hcy+Cbl+ (2.45 +/- 0.35), compared to cells cultivated in Hcy+Met+ (0.18 +/- 0.01, p<0.001), in Met+ Cbl+ (1.60 +/- 0.06, p<0.05), and in Met+ (0.40 +/- 0.05, p<0.001), suggesting an adaptation of the cells to requirement in synthesized methionine. The mRNA level of MTR in Hcy+ Cbl+ and Hcy+Met+ (2.82 +/-0.49 and 3.33 +/- 0.48 AU, respectively ) was about 2.5 / 3.0-fold higher than that in Met+ Cbl+ and in Met+ (1.00 +/-0.13 and 1.20 +/-0.20 AU, respectively, p<0.001). CONCLUSION: Methionine synthase expression of Caco-2 cell is under a transcriptional control influenced by Hcy. |
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Authors:
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Sandrine Ortiou; Jean-Marc Alberto; Jean-Louis Guéant; Marc Merten |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology Volume: 14 ISSN: 1015-8987 ISO Abbreviation: Cell. Physiol. Biochem. Publication Date: 2004 |
Date Detail:
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Created Date: 2004-08-20 Completed Date: 2005-02-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9113221 Medline TA: Cell Physiol Biochem Country: Switzerland |
Other Details:
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Languages: eng Pagination: 407-14 Citation Subset: IM |
Affiliation:
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Laboratoire de Pathologie Cellulaire et Moléculaire en Nutrition, Equipe INSERM 0014, Faculté de Médecine, Vandoeuvre-les-Nancy, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase
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analysis,
biosynthesis,
genetics* Adenocarcinoma / chemistry, enzymology, genetics* Caco-2 Cells Gene Expression Regulation, Neoplastic* Homocysteine / pharmacology, physiology* Humans Methionine / pharmacology, physiology RNA, Messenger / analysis, metabolism Transcription, Genetic / genetics Up-Regulation* Vitamin B 12 / pharmacology, physiology |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 454-28-4/Homocysteine; 63-68-3/Methionine; 68-19-9/Vitamin B 12; EC 2.1.1.13/5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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