| Homocysteine enhances LDL fatty acid peroxidation, promoting microalbuminuria in type 2 diabetes. | |
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MedLine Citation:
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PMID: 18753419 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: We aimed to establish the relationship between glycated haemoglobin (HbA(1c)), hypertension and microalbuminuria onset in type 2 diabetes. We also intended to ascertain the metabolic action of homocysteine on LDL fatty acids and on renal function. METHODS: The study was carried out on 200 patients with type 2 diabetes and 200 healthy subjects. HbA(1c), apolipoprotein B (apo B) and microalbuminuria were measured using immunoturbidimetric methods. Cholesterol, peroxide, urea and uric acid were assayed using colorimetric methods. Creatinine clearance was calculated using the Cockroft-Gault equation. Homocysteine was measured by immunological fluorescence polarization. LDL fatty acids were quantified by gas chromatography. RESULTS: Creatinine and microalbuminuria significantly increased in type 2 diabetes when compared with controls. Microalbuminuria was significantly correlated with HbA(1c) and with the presence of high blood pressure. Homocysteinaemia significantly correlated with creatinine clearance in diabetes. Linoleic acid (C18:2omega6) did not differ between groups. C18:2omega6/C18:3omega3 ratio was three times higher in diabetics than in controls. Total saturated fatty acids, homocysteine, H(2)O(2) and LDL-thiobarbituric reactive substances significantly increased in microalbuminuric when compared with normoalbuminuric diabetes. Total polyunsaturated fatty acids, arachidonic acid (C20:4omega6), LDL-cholesterol, apo B and creatinine clearance significantly decreased in microalbuminuric when compared with normoalbuminuric diabetes. CONCLUSION: Microalbuminuria onset is associated with renal protein oxidation that is preceded by LDL fatty acid oxidation. The latter is initiated by H(2)O(2) produced from an auto-oxidation of homocysteine and increased metabolism of arachidonic acid towards its pro-inflammatory eicosanoids. An oxidative stress state is the common ground of diffused vasculopathy. |
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Authors:
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Asma Kassab; Thouraya Ajmi; Manel Issaoui; Larbi Chaeib; Abdelhedi Miled; Mohamed Hammami |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Annals of clinical biochemistry Volume: 45 ISSN: 0004-5632 ISO Abbreviation: Ann. Clin. Biochem. Publication Date: 2008 Sep |
Date Detail:
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Created Date: 2008-08-28 Completed Date: 2008-11-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0324055 Medline TA: Ann Clin Biochem Country: England |
Other Details:
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Languages: eng Pagination: 476-80 Citation Subset: IM |
Affiliation:
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Biochemistry Laboratory, CHU F Hached Sousse, Tunisia. kassab.asma@laposte.net |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aged Albuminuria / blood*, complications* Cardiovascular Diseases / blood, diagnosis Diabetes Mellitus, Type 2 / blood*, complications* Fatty Acids / chemistry* Female Homocysteine / chemistry* Humans Lipoproteins, LDL / chemistry* Male Middle Aged Oxidants / chemistry Peroxides Risk Factors Thiobarbituric Acid Reactive Substances |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Lipoproteins, LDL; 0/Oxidants; 0/Peroxides; 0/Thiobarbituric Acid Reactive Substances; 454-28-4/Homocysteine |
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