Document Detail

Homocysteine enhances LDL fatty acid peroxidation, promoting microalbuminuria in type 2 diabetes.
MedLine Citation:
PMID:  18753419     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: We aimed to establish the relationship between glycated haemoglobin (HbA(1c)), hypertension and microalbuminuria onset in type 2 diabetes. We also intended to ascertain the metabolic action of homocysteine on LDL fatty acids and on renal function. METHODS: The study was carried out on 200 patients with type 2 diabetes and 200 healthy subjects. HbA(1c), apolipoprotein B (apo B) and microalbuminuria were measured using immunoturbidimetric methods. Cholesterol, peroxide, urea and uric acid were assayed using colorimetric methods. Creatinine clearance was calculated using the Cockroft-Gault equation. Homocysteine was measured by immunological fluorescence polarization. LDL fatty acids were quantified by gas chromatography. RESULTS: Creatinine and microalbuminuria significantly increased in type 2 diabetes when compared with controls. Microalbuminuria was significantly correlated with HbA(1c) and with the presence of high blood pressure. Homocysteinaemia significantly correlated with creatinine clearance in diabetes. Linoleic acid (C18:2omega6) did not differ between groups. C18:2omega6/C18:3omega3 ratio was three times higher in diabetics than in controls. Total saturated fatty acids, homocysteine, H(2)O(2) and LDL-thiobarbituric reactive substances significantly increased in microalbuminuric when compared with normoalbuminuric diabetes. Total polyunsaturated fatty acids, arachidonic acid (C20:4omega6), LDL-cholesterol, apo B and creatinine clearance significantly decreased in microalbuminuric when compared with normoalbuminuric diabetes. CONCLUSION: Microalbuminuria onset is associated with renal protein oxidation that is preceded by LDL fatty acid oxidation. The latter is initiated by H(2)O(2) produced from an auto-oxidation of homocysteine and increased metabolism of arachidonic acid towards its pro-inflammatory eicosanoids. An oxidative stress state is the common ground of diffused vasculopathy.
Asma Kassab; Thouraya Ajmi; Manel Issaoui; Larbi Chaeib; Abdelhedi Miled; Mohamed Hammami
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Annals of clinical biochemistry     Volume:  45     ISSN:  0004-5632     ISO Abbreviation:  Ann. Clin. Biochem.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-08-28     Completed Date:  2008-11-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0324055     Medline TA:  Ann Clin Biochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  476-80     Citation Subset:  IM    
Biochemistry Laboratory, CHU F Hached Sousse, Tunisia.
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MeSH Terms
Albuminuria / blood*,  complications*
Cardiovascular Diseases / blood,  diagnosis
Diabetes Mellitus, Type 2 / blood*,  complications*
Fatty Acids / chemistry*
Homocysteine / chemistry*
Lipoproteins, LDL / chemistry*
Middle Aged
Oxidants / chemistry
Risk Factors
Thiobarbituric Acid Reactive Substances
Reg. No./Substance:
0/Fatty Acids; 0/Lipoproteins, LDL; 0/Oxidants; 0/Peroxides; 0/Thiobarbituric Acid Reactive Substances; 454-28-4/Homocysteine

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