| Homo-D: -lactic acid production from mixed sugars using xylose-assimilating operon-integrated Lactobacillus plantarum. | |
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MedLine Citation:
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PMID: 21643702 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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In order to achieve efficient D: -lactic acid fermentation from a mixture of xylose and glucose, the xylose-assimilating xylAB operon from Lactobacillus pentosus (PXylAB) was introduced into an L: -lactate dehydrogenase gene (ldhL1)-deficient Lactobacillus plantarum (ΔldhL1-xpk1::tkt-Δxpk2) strain in which the phosphoketolase 1 gene (xpk1) was replaced with the transketolase gene (tkt) from Lactococcus lactis, and the phosphoketolase 2 (xpk2) gene was deleted. Two copies of xylAB introduced into the genome significantly improved the xylose fermentation ability, raising it to the same level as that of ΔldhL1-xpk1::tkt-Δxpk2 harboring a xylAB operon-expressing plasmid. Using the two-copy xylAB integrated strain, successful homo-D: -lactic acid production was achieved from a mixture of 25 g/l xylose and 75 g/l glucose without carbon catabolite repression. After 36-h cultivation, 74.2 g/l of lactic acid was produced with a high yield (0.78 g per gram of consumed sugar) and an optical purity of D: -lactic acid of 99.5%. Finally, we successfully demonstrated homo-D: -lactic acid fermentation from a mixture of three kinds of sugar: glucose, xylose, and arabinose. This is the first report that describes homo-D: -lactic acid fermentation from mixed sugars without carbon catabolite repression using the xylose-assimilating pathway integrated into lactic acid bacteria. |
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Authors:
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Shogo Yoshida; Kenji Okano; Tsutomu Tanaka; Chiaki Ogino; Akihiko Kondo |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-6-4 |
Journal Detail:
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Title: Applied microbiology and biotechnology Volume: - ISSN: 1432-0614 ISO Abbreviation: - Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-6-6 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8406612 Medline TA: Appl Microbiol Biotechnol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1, Rokkodai, Nada, Kobe, 657-8501, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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