| Homer1 regulates the susceptibility to TRAIL. | |
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MedLine Citation:
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PMID: 19376111 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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TRAIL is an apoptotic cell death-inducing ligand that belongs to a TNF superfamily. To identify the regulators that govern the susceptibility to TRAIL, TRAIL-resistant HeLa (TR) cells were established by repeatedly treating HeLa cells with TRAIL. Here we showed that scaffolding protein Homer1 plays a decisive role in regulating the apoptotic susceptibility to TRAIL. TR cells showing the normal susceptibility to FasL and chemotherapeutic agent etoposide expressed the lower protein levels of Homer1 than parental HeLa cells. They showed the delayed activation of caspases-8, Bid cleavage and Bax translocation to mitochondria in response to TRAIL. Reconstitution of Homer1 expression in TR cells significantly restored the susceptibility to TRAIL. In addition, knock-down of Homer1 using interfering shRNA in parental HeLa cells lost the susceptibility to TRAIL. Together, our data indicate that Homer1 plays a critical role in determining the apoptotic susceptibility to TRAIL. |
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Authors:
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Jin Na Shin; Sujan Piya; Cheol-Won Yun; Dai-Wu Seol; Young-Woo Seo; Ae Ran Moon; Tae-Hyoung Kim |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-04-17 |
Journal Detail:
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Title: Experimental cell research Volume: 315 ISSN: 1090-2422 ISO Abbreviation: Exp. Cell Res. Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-06-15 Completed Date: 2009-07-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0373226 Medline TA: Exp Cell Res Country: United States |
Other Details:
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Languages: eng Pagination: 2249-55 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Chosun University School of Medicine, 375 Seosuk-Dong, Dong-Gu, Gwangju, 501-759, Republic of Korea. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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physiology* Carrier Proteins / genetics, metabolism* Gene Expression Profiling Hela Cells Humans Mitochondria / metabolism Oligonucleotide Array Sequence Analysis RNA Interference TNF-Related Apoptosis-Inducing Ligand / genetics, metabolism* bcl-2-Associated X Protein / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Homer protein; 0/TNF-Related Apoptosis-Inducing Ligand; 0/bcl-2-Associated X Protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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