Document Detail


Hollow-fiber ultrafiltration for the concentration and simultaneous recovery of multiple pathogens in contaminated foods.
MedLine Citation:
PMID:  20003737     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated the possibility of using hollow-fiber ultrafiltration (HUF) for the simultaneous recovery of multiple microorganisms in food samples. MS2 bacteriophage, E. coli, Bacillus subtilis spores, and murine norovirus (MNV) were each inoculated into 5 liters of either distilled water (DW) or glycine elution buffer and then concentrated using hollow-fiber polysulfone ultrafilters. The resulting concentrates were further analyzed by either cultivation or TaqMan real-time reverse transcription PCR assay. The overall average recovery rates were 7.1% in DW and 17.1% in glycine elution buffer. When the virus, vegetative bacteria, and bacterial spores were simultaneously inoculated into DW, glycine, or Tris-HCl elution buffers, on average 16.8% of inoculated microorganisms were recovered by HUF. The addition of 3% beef extract blocking buffer to HUF increased the total recovery rate to 46.1%, with incremental recovery rates increasing sharply for B. subtilis spores and MNV. Use of HUF resulted in E. coli recovery rates of 68.0% on lettuce and 66.2% on ham and MNV recovery rates of 1.5% on lettuce and 5.8% on ham. Our study demonstrates that HUF can be effective at simultaneously recovering and concentrating diverse bacterial and viral pathogens from foods.
Authors:
Hee-Yeon Kim; Hyeun-Jin Park; Gwangpyo Ko
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of food protection     Volume:  72     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-12-16     Completed Date:  2010-01-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2547-52     Citation Subset:  IM    
Affiliation:
Department of Environmental Health and Institute of Health and Environment, School of Public Health, Seoul National University, Seoul, Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacillus subtilis / isolation & purification*
Cattle
Culture
Escherichia coli / isolation & purification*
Food Microbiology*
Glycine
Lettuce / microbiology
Levivirus / isolation & purification*
Meat / microbiology
Norovirus / isolation & purification*
Spores, Bacterial / isolation & purification
Swine
Ultrafiltration / methods*
Water
Water Microbiology
Chemical
Reg. No./Substance:
56-40-6/Glycine; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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