Document Detail

Hollow-fiber blood-dialysis membranes: superoxide generation, permeation, and dismutation measured by chemiluminescence.
MedLine Citation:
PMID:  16362524     Owner:  NLM     Status:  MEDLINE    
The interaction of blood with a material surface results in activation of the body's humoral immune system and the generation of reactive oxygen species (ROS). It has recently become clear that ROS are central to the pathology of many diseases. In this study, we evaluated the superoxide generation, permeation, and dismutation in hollow-fiber dialysis membranes by using 2-methyl-6-p-methoxyphenylethynyl-imidazopyrazinone (MPEC) as a superoxide-reactive chemiluminescence producer and an optical fiber probe to detect the resulting chemiluminescence in the hollow fiber lumen. We measured the superoxide generated when bovine blood leukocytes were brought into contact with dialysis membranes. Superoxide permeation was determined by measuring MPEC chemiluminescence in the hollow fiber lumen using an optical fiber probe. Additionally, superoxide dismutation was evaluated by examining the difference in superoxide permeability for membranes with and without vitamin E coating. Superoxide generation varies for different membrane materials, depending on the membrane's biocompatibility. Superoxide permeability depends on the diffusive permeability of membranes. No marked decrease in superoxide permeability was observed among membrane materials. The superoxide permeability of vitamin E-coated membrane was smaller than that of uncoated membrane. The antioxidant property of vitamin E-coated membranes is hence effective in causing superoxide dismutation.
Ken-ichiro Yamamoto; Kazuyoshi Kobayashi; Kosuke Endo; Takehiro Miyasaka; Seiichi Mochizuki; Fukashi Kohori; Kiyotaka Sakai
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article    
Journal Detail:
Title:  Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs     Volume:  8     ISSN:  1434-7229     ISO Abbreviation:  -     Publication Date:  2005  
Date Detail:
Created Date:  2005-12-19     Completed Date:  2006-03-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9815648     Medline TA:  J Artif Organs     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  257-62     Citation Subset:  IM    
Department of Chemical Engineering, 21st Century COE Program, Waseda University, 3-4-1 Okubo, Tokyo 169-8555, Japan.
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MeSH Terms
Antioxidants / pharmacology
Coated Materials, Biocompatible
Free Radical Scavengers / metabolism
Luminescent Measurements
Membranes, Artificial*
Renal Dialysis / instrumentation*
Superoxide Dismutase / metabolism
Superoxides / metabolism*
Vitamin E / pharmacology
Reg. No./Substance:
0/Antioxidants; 0/Coated Materials, Biocompatible; 0/Free Radical Scavengers; 0/Membranes, Artificial; 11062-77-4/Superoxides; 1406-18-4/Vitamin E; EC Dismutase

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