Document Detail

Holding bovine oocytes in the absence of maturation inhibitors: kinetics of in vitro maturation and effect on blastocyst development after in vitro fertilization.
MedLine Citation:
PMID:  18644621     Owner:  NLM     Status:  MEDLINE    
Holding immature oocytes before maturation simplifies the transport of oocytes and aids in scheduling later manipulations. We examined the effect of holding bovine oocytes in the absence of meiotic inhibitors on their subsequent meiotic and developmental competence. Oocytes were matured immediately after recovery (control) or were held in a mixture of 40% TCM 199 with Earle's salts, 40% TCM 199 with Hanks' salts, and 20% FBS, at room temperature for 16 to 18 h (EH-held) and then matured. Chromatin status was determined at 0, 10, 14, 18, and 22 h of maturation culture. Oocytes were fertilized in vitro after either 18 or 22-24h maturation. The EH treatment maintained oocytes at the germinal vesicle stage (79.3%, vs. 87.7% for control oocytes at 0 h; P>0.05). Upon culture, held oocytes matured more quickly than did control oocytes. The proportions of mature oocytes were not significantly different between groups at 18 h (EH-held, 80.6% and control, 79.3%); however, after 22 h significantly more EH-held than control oocytes had degenerated (24.1% vs. 4.5%, P<0.0001). Blastocyst development was similar between groups for oocytes fertilized after 18 h maturation (EH-held, 29.6% and control, 27.8%). When oocytes were fertilized after 22-24h maturation, EH-held oocytes yielded lower blastocyst development than did control oocytes (16.5% vs. 29.3%, P<0.05). In conclusion, bovine oocytes may be effectively held in the EH treatment before maturation without adversely affecting meiotic or developmental competence. However, holding affects the kinetics of maturation and this must be taken into account when subsequent manipulations are performed.
H Alm; Y H Choi; L Love; B Heleil; H Torner; K Hinrichs
Publication Detail:
Type:  Journal Article     Date:  2008-07-21
Journal Detail:
Title:  Theriogenology     Volume:  70     ISSN:  0093-691X     ISO Abbreviation:  Theriogenology     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-09-15     Completed Date:  2008-12-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1024-9     Citation Subset:  IM    
FBN Research Institute for the Biology of Farm Animals, 18196 Dummerstorf, Germany.
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MeSH Terms
Blastocyst / cytology*
Embryo Culture Techniques / veterinary*
Embryonic Development / physiology
Fertilization in Vitro / veterinary*
Oocytes / physiology*
Time Factors

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