Document Detail

Hoechst 33258: a fluorescent nuclear counterstain suitable for double-labelling immunofluorescence.
MedLine Citation:
PMID:  6193197     Owner:  NLM     Status:  MEDLINE    
Counterstaining with the chromosomal dye Hoechst 33258 is a simple procedure that provides an excellent general purpose nuclear counterstain for immunofluorescent work. Benefits from its use include the ready identification and orientation of structures, differentiation of lymphoid and non-lymphoid cells and easy assessment of frequency in specifically labelled cellular subpopulations. It can be used at the same time as FITC and TRITC double-labelling immunofluorescence.
D R Bainbridge; M M Macey
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of immunological methods     Volume:  62     ISSN:  0022-1759     ISO Abbreviation:  J. Immunol. Methods     Publication Date:  1983 Aug 
Date Detail:
Created Date:  1983-10-08     Completed Date:  1983-10-08     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  1305440     Medline TA:  J Immunol Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  193-5     Citation Subset:  IM    
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MeSH Terms
Benzimidazoles / diagnostic use*
Bisbenzimidazole / diagnostic use*
Cell Nucleus / ultrastructure
Fluorescent Antibody Technique*
Microscopy, Fluorescence / methods*
Reg. No./Substance:
0/Benzimidazoles; 23491-44-3/Bisbenzimidazole

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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