Document Detail

Hodgkin and Reed-Sternberg-like cells in B-cell chronic lymphocytic leukemia represent the outgrowth of single germinal-center B-cell-derived clones: potential precursors of Hodgkin and Reed-Sternberg cells in Hodgkin's disease.
MedLine Citation:
PMID:  10648418     Owner:  NLM     Status:  MEDLINE    
In rare cases of B-cell chronic lymphocytic leukemia (B-CLL), large cells morphologically similar to or indistinguishable from Hodgkin/Reed-Sternberg (HRS) cells of Hodgkin's disease (HD) can be found in a background of otherwise typical B-CLL. To test these HRS-like cells for a potential clonal relationship to the B-CLL cells, single cells were micromanipulated from immunostained tissue sections, and rearranged immunoglobulin genes were amplified from HRS-like cells and B-CLL cells and sequenced. The same variable (V) gene rearrangements with shared and distinct somatic mutations were found in HRS-like and B-CLL cells from 1 patient, which indicates derivation of these cells from 2 distinct members of a germinal-center B-cell clone. Separate clonal V gene rearrangements were amplified from HRS-like and B-CLL cells from 2 other patients, showing concomitant presence of 2 distinct expanded B-cell clones. Epstein-Barr virus (EBV) was detected in the HRS-like cells of these 2 latter cases, indicating clonal expansion of an EBV-harboring B cell in the setting of B-CLL. There is evidence that HRS-like cells in B-CLL, like HRS cells in HD, derive from germinal-center B cells. In all cases, somatic mutations have been detected in the rearranged V genes of the HRS-like cells, and in 1 of the EBV-positive HRS-like cell clones, somatic mutations rendered an originally functional V gene rearrangement nonfunctional. We speculate that the HRS-like cells in B-CLL represent potential precursors for HRS cells causing HD.
H Kanzler; R Küppers; S Helmes; H H Wacker; A Chott; M L Hansmann; K Rajewsky
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Publication Detail:
Type:  Case Reports; Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Blood     Volume:  95     ISSN:  0006-4971     ISO Abbreviation:  Blood     Publication Date:  2000 Feb 
Date Detail:
Created Date:  2000-02-24     Completed Date:  2000-02-24     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7603509     Medline TA:  Blood     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1023-31     Citation Subset:  AIM; IM    
Institute for Genetics, Department of Internal Medicine, University of Cologne, Cologne, Germany.
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MeSH Terms
Aged, 80 and over
B-Lymphocytes / pathology*
Base Sequence
Cell Transformation, Neoplastic
Cell Transformation, Viral
Clone Cells / pathology
DNA, Neoplasm / genetics
Embryonal Carcinoma Stem Cells
Epstein-Barr Virus Infections / pathology
Fatal Outcome
Gene Rearrangement, B-Lymphocyte, Heavy Chain
Genes, Immunoglobulin
Germinal Center / pathology*
Herpesvirus 4, Human / isolation & purification
Hodgkin Disease / pathology*
Immunoglobulin Variable Region / genetics
Leukemia, Lymphocytic, Chronic, B-Cell / genetics,  pathology*,  virology
Middle Aged
Molecular Sequence Data
Neoplastic Stem Cells / pathology*
Polymerase Chain Reaction
Reed-Sternberg Cells / pathology*
Sequence Alignment
Sequence Homology, Nucleic Acid
Tumor Virus Infections / pathology
Reg. No./Substance:
0/DNA, Neoplasm; 0/Immunoglobulin Variable Region

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