|Hodgkin and Reed-Sternberg-like cells in B-cell chronic lymphocytic leukemia represent the outgrowth of single germinal-center B-cell-derived clones: potential precursors of Hodgkin and Reed-Sternberg cells in Hodgkin's disease.|
|PMID: 10648418 Owner: NLM Status: MEDLINE|
|In rare cases of B-cell chronic lymphocytic leukemia (B-CLL), large cells morphologically similar to or indistinguishable from Hodgkin/Reed-Sternberg (HRS) cells of Hodgkin's disease (HD) can be found in a background of otherwise typical B-CLL. To test these HRS-like cells for a potential clonal relationship to the B-CLL cells, single cells were micromanipulated from immunostained tissue sections, and rearranged immunoglobulin genes were amplified from HRS-like cells and B-CLL cells and sequenced. The same variable (V) gene rearrangements with shared and distinct somatic mutations were found in HRS-like and B-CLL cells from 1 patient, which indicates derivation of these cells from 2 distinct members of a germinal-center B-cell clone. Separate clonal V gene rearrangements were amplified from HRS-like and B-CLL cells from 2 other patients, showing concomitant presence of 2 distinct expanded B-cell clones. Epstein-Barr virus (EBV) was detected in the HRS-like cells of these 2 latter cases, indicating clonal expansion of an EBV-harboring B cell in the setting of B-CLL. There is evidence that HRS-like cells in B-CLL, like HRS cells in HD, derive from germinal-center B cells. In all cases, somatic mutations have been detected in the rearranged V genes of the HRS-like cells, and in 1 of the EBV-positive HRS-like cell clones, somatic mutations rendered an originally functional V gene rearrangement nonfunctional. We speculate that the HRS-like cells in B-CLL represent potential precursors for HRS cells causing HD.|
|H Kanzler; R Küppers; S Helmes; H H Wacker; A Chott; M L Hansmann; K Rajewsky|
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|Type: Case Reports; Comparative Study; Journal Article; Research Support, Non-U.S. Gov't|
|Title: Blood Volume: 95 ISSN: 0006-4971 ISO Abbreviation: Blood Publication Date: 2000 Feb|
|Created Date: 2000-02-24 Completed Date: 2000-02-24 Revised Date: 2008-11-21|
Medline Journal Info:
|Nlm Unique ID: 7603509 Medline TA: Blood Country: UNITED STATES|
|Languages: eng Pagination: 1023-31 Citation Subset: AIM; IM|
|Institute for Genetics, Department of Internal Medicine, University of Cologne, Cologne, Germany.|
|APA/MLA Format Download EndNote Download BibTex|
Aged, 80 and over
B-Lymphocytes / pathology*
Cell Transformation, Neoplastic
Cell Transformation, Viral
Clone Cells / pathology
DNA, Neoplasm / genetics
Embryonal Carcinoma Stem Cells
Epstein-Barr Virus Infections / pathology
Gene Rearrangement, B-Lymphocyte, Heavy Chain
Germinal Center / pathology*
Herpesvirus 4, Human / isolation & purification
Hodgkin Disease / pathology*
Immunoglobulin Variable Region / genetics
Leukemia, Lymphocytic, Chronic, B-Cell / genetics, pathology*, virology
Molecular Sequence Data
Neoplastic Stem Cells / pathology*
Polymerase Chain Reaction
Reed-Sternberg Cells / pathology*
Sequence Homology, Nucleic Acid
Tumor Virus Infections / pathology
|0/DNA, Neoplasm; 0/Immunoglobulin Variable Region|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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