Document Detail

Histopathologic findings and frequency of clonality detected by the polymerase chain reaction in ocular adnexal lymphoproliferative lesions.
MedLine Citation:
PMID:  8933515     Owner:  NLM     Status:  MEDLINE    
We report the reclassification according to recently described histologic categories of 48 patients with ocular adnexal lymphoproliferative lesions with long-term follow-up (mean, 8.1 yr). We used available formalin-fixed, paraffin-embedded, and frozen tissues to assess the frequency of immunoglobulin heavy chain gene rearrangement detectable by polymerase chain reaction in these lesions. We reviewed patient records, obtained follow-up data, and examined hematoxylin- and eosin-stained slides. DNA extracted from tissues was amplified with consensus V- and J-region primers to detect immunoglobulin heavy chain gene rearrangement. We examined 28 orbital, 10 lacrimal, and 10 conjunctival lesions, of which 2 lesions were lymphoid hyperplasias, 3 were indeterminate, and 43 were lymphomas. Of the 44 patients with follow-up, systemic lymphoma developed in 24 (55%), of whom 11 died of the disease, and 6 are alive with disease. Thirty-one patients had sufficient DNA for polymerase chain reaction analysis; 9 specimens were nonclonal, 21 were clonal, and 1 failed to amplify. The nonclonal lesions included one hyperplasia, one indeterminate lesion, and seven lymphomas; two of these patients died of the disease, and one is alive with disease. The clonal lesions included 1 indeterminate lesion and 20 lymphomas. Systemic lymphomas developed in 16 patients; 8 died of the disease, and 4 are alive with disease. Of the lesions histologically classified as lymphoma, 74% were clonal. We conclude that most ocular adnexal lymphoproliferative lesions can be histologically classified as lymphomas, that systemic lymphoma will develop in at least 50% of these patients if they are followed for sufficient time, and that most lesions classified as lymphomas will be clonal using polymerase chain reaction techniques. Lack of amplification using a consensus primer strategy may account for the inability to detect clonality by polymerase chain reaction in some histologically identified lymphomas.
V A White; R D Gascoyne; B K McNeil; W Y Chang; L V Brewer; J Rootman
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc     Volume:  9     ISSN:  0893-3952     ISO Abbreviation:  Mod. Pathol.     Publication Date:  1996 Nov 
Date Detail:
Created Date:  1997-02-13     Completed Date:  1997-02-13     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8806605     Medline TA:  Mod Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1052-61     Citation Subset:  IM    
Department of Pathology, Vancouver Hospital and Health Sciences Centre, British Columbia, Canada.
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MeSH Terms
Aged, 80 and over
Clone Cells
Conjunctival Neoplasms / chemistry,  classification,  pathology*
DNA Primers / chemistry
DNA, Neoplasm / analysis
Gene Rearrangement, B-Lymphocyte, Heavy Chain / genetics
Lacrimal Apparatus Diseases / classification,  pathology*
Lymphoma / chemistry,  classification,  pathology*
Lymphoma, B-Cell, Marginal Zone / chemistry,  classification,  pathology*
Middle Aged
Orbital Neoplasms / chemistry,  classification,  pathology*
Polymerase Chain Reaction*
Reg. No./Substance:
0/DNA Primers; 0/DNA, Neoplasm

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