| Histone deacetylase inhibitors prime medulloblastoma cells for chemotherapy-induced apoptosis by enhancing p53-dependent Bax activation. | |
| | |
MedLine Citation:
|
PMID: 21562496 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Despite aggressive therapies, the prognosis of children with high-risk medulloblastoma is still poor, thus underscoring the need to develop novel treatment strategies. Here, we report that histone deacetylase inhibitors (HDACI), that is, MS-275, valproic acid or SAHA, provide a novel strategy for sensitization of medulloblastoma to DNA-damaging drugs such as Doxorubicin, VP16 and Cisplatin by promoting p53-dependent, mitochondrial apoptosis. Mechanistic studies reveal that single-agent treatment with MS-275 causes acetylation of the non-histone protein Ku70, an event reported to release Bax from Ku70, whereas DNA-damaging drugs trigger p53 acetylation and accumulation. Combined treatment with MS-275 and Doxorubicin or VP16 cooperates to promote binding of p53 to Bax and p53-dependent Bax activation, resulting in enhanced loss of mitochondrial membrane potential, cytochrome c release and caspase-dependent apoptosis. Overexpression of Bcl-2 almost completely abolishes the MS-275-mediated chemosensitization, underlining the importance of the mitochondrial pathway for inducing apoptosis. Also, MS-275 cooperates with chemotherapeutics to inhibit long-term clonogenic survival. Most importantly, MS-275 increases chemotherapeutic drug-induced apoptosis in primary medulloblastoma samples, and cooperates with Doxorubicin to suppress medulloblastoma growth in an in vivo model, which underscores the clinical relevance of the findings. Thus, HDACI such as MS-275 present a promising approach for chemosensitization of medulloblastoma by enhancing mitochondrial apoptosis in a p53-dependent manner. These findings have important clinical implications for the design of experimental treatment protocols for medulloblastoma. |
| | |
Authors:
|
S Häcker; S Karl; I Mader; S Cristofanon; T Schweitzer; J Krauss; S Rutkowski; K-M Debatin; S Fulda |
Related Documents
:
|
19538496 - Regulatory t cells and asthma. 20591166 - Azithromycin attenuates airway inflammation in a mouse model of viral bronchiolitis. 19752226 - Regulatory t cells negatively regulate neovasculature of airway remodeling via dll4-not... 19648516 - A hypothesis for the initiation of copd. 10328196 - Systemic toxicity following administration of sirolimus (formerly rapamycin) for psoria... 20722596 - How do malaria parasites activate dendritic cells? |
Publication Detail:
|
Type: Journal Article Date: 2011-01-17 |
Journal Detail:
|
Title: Oncogene Volume: 30 ISSN: 1476-5594 ISO Abbreviation: Oncogene Publication Date: 2011 May |
Date Detail:
|
Created Date: 2011-05-12 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8711562 Medline TA: Oncogene Country: England |
Other Details:
|
Languages: eng Pagination: 2275-81 Citation Subset: IM |
Affiliation:
|
University Children's Hospital, Ulm, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Structure and function of a membrane component SecDF that enhances protein export.
Next Document: Visit-to-visit variability in blood pressure over a 1-year period is a marker of left ventricular di...