| Histone deacetylase inhibitor trichostatin A induced caspase-independent apoptosis in human gastric cancer cell. | |
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MedLine Citation:
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PMID: 18167185 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Histone deacetylase inhibitors (HDACIs) have been reported to induce apoptosis in cancer cells. The effects of trichostatin A (TSA) on gastric cancer cells have not been well characterized. This study was aimed to explore the effects and mechanisms of TSA on human gastric cancer SGC-7901 cells. METHODS: The cells were treated with TSA and analyzed by cell proliferation assay, Western blot, TUNEL assay, flow cytometry by fluorescein isothiocyanate (FITC) conjugated with Annexin V and PI staining, immunofluorescence analysis, analysis of subcellular fractionation, gene chips and real time polymerase chain reaction (PCR). RESULTS: TSA could inhibit cell growth and induced apoptosis in gastric cancer SGC-7901 cells through the regulation of apoptosis-related genes, such as Bcl-2, Bax and survivin. Further study indicated that the pan-caspase inhibitor z-VAD-fmk did not inhibit the apoptosis induced by TSA, and we did not observe the cleavage of poly ADP ribose polymerase (PARP) after TSA treatment too. In addition, apoptosis inducing factor (AIF) and EndoG were found to translocate from mitochondria to nucleus in the immunofluorescence assay and the Western analysis of subcellular fractionation confirmed the result of immunofluorescence assay. CONCLUSIONS: The apoptosis induced by TSA in gastric cancer SGC-7901 cells involves a caspase-independent pathway. |
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Authors:
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Zhi-qun Wu; Rui Zhang; Connie Chao; Ji-feng Zhang; Yuan-qiang Zhang |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Chinese medical journal Volume: 120 ISSN: 0366-6999 ISO Abbreviation: Chin. Med. J. Publication Date: 2007 Dec |
Date Detail:
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Created Date: 2008-01-02 Completed Date: 2008-02-05 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 7513795 Medline TA: Chin Med J (Engl) Country: China |
Other Details:
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Languages: eng Pagination: 2112-8 Citation Subset: IM |
Affiliation:
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Center of Cancer Therapy, Tangdu Hospital, The Fourth Military Medical University, Xi'an, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects* Caspases / physiology* Cell Line, Tumor Enzyme Inhibitors / pharmacology* Gene Expression Profiling Histone Deacetylase Inhibitors* Humans Hydroxamic Acids / pharmacology* Microtubule-Associated Proteins / analysis Neoplasm Proteins / analysis Proto-Oncogene Proteins c-bcl-2 / analysis Stomach Neoplasms / drug therapy*, pathology Tumor Suppressor Protein p53 / analysis, physiology bcl-2-Associated X Protein / analysis |
| Chemical | |
Reg. No./Substance:
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0/BIRC5 protein, human; 0/Enzyme Inhibitors; 0/Histone Deacetylase Inhibitors; 0/Hydroxamic Acids; 0/Microtubule-Associated Proteins; 0/Neoplasm Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/Tumor Suppressor Protein p53; 0/bcl-2-Associated X Protein; 58880-19-6/trichostatin A; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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