Document Detail


Histone H4 post-translational modifications in chordate mitotic and endoreduplicative cell cycles.
MedLine Citation:
PMID:  15937898     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Histone post-translational modifications mark distinct structural and functional chromatin states but little is known of their involvement in the progression of different cell cycle types across phylogeny. We compared temporal and spatial dynamics of histone H4 post-translational modifications during both mitotic and endoreduplicative cycles of the urochordate, Oikopleura dioica, and proliferating mammalian cells. Endocycling cells showed no signs of chromosome condensation or entry into mitosis. They exhibited an evolution of replication patterns indicative of reduced chromatin compartmentalization relative to proliferating mammalian cells. In the latter cells, published cell cycle profiles of histone H4 acetylated at lysine 16 (H4AcK16) or dimethylated at lysine 20 (H4Me2K20) are disputed. Our results, using different, widely used H4AcK16 antibodies, revealed significant antibody-specific discrepancies in spatial and temporal cell cycle regulation of this modification, with repercussions for interpretation of previous immunofluorescence and immunoprecipitation data based on these reagents. On the other hand, three different antibodies to H4Me2K20 revealed similar cell cycle profiles of this modification that were conserved throughout the mitotic cell cycle in urochordate and mammalian cells, with accumulation at mitosis and a decrease during S-phase. H4Me2K20 also cycled in endocycles, indicating that dynamics of this modification are not strictly constrained by the mitotic phase of the cell cycle and suggesting additional roles during G- and S-phase progression. This article contains Supplementary Material available at http://www.mrw.interscience.wiley.com/suppmat/0730-2312/suppmat/2005/95/spada.html.
Authors:
Fabio Spada; Mariacristina Chioda; Eric M Thompson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cellular biochemistry     Volume:  95     ISSN:  0730-2312     ISO Abbreviation:  J. Cell. Biochem.     Publication Date:  2005 Aug 
Date Detail:
Created Date:  2005-07-26     Completed Date:  2005-10-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8205768     Medline TA:  J Cell Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  885-901     Citation Subset:  IM    
Copyright Information:
(c) 2005 Wiley-Liss, Inc.
Affiliation:
Sars International Centre for Marine Molecular Biology, Bergen High Technology Centre, Thormølensgt. 55, N-5008 Bergen, Norway. f.spada@lmu.edu
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MeSH Terms
Descriptor/Qualifier:
Acetylation
Animals
Chromatin / metabolism*
G1 Phase / physiology*
Gene Expression Regulation
Hela Cells
Histones / genetics,  metabolism*
Humans
Immunoblotting
Immunoprecipitation
Mice
Mitosis*
NIH 3T3 Cells
Protein Processing, Post-Translational
S Phase / physiology*
Urochordata
Chemical
Reg. No./Substance:
0/Chromatin; 0/Histones

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