| Histone H3K27me3 demethylases KDM6A and KDM6B modulate definitive endoderm differentiation from human ESCs by regulating WNT signaling pathway. | |
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MedLine Citation:
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PMID: 22907667 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Definitive endoderm differentiation is crucial for generating respiratory and gastrointestinal organs including pancreas and liver. However, whether epigenetic regulation contributes to this process is unknown. Here, we show that the H3K27me3 demethylases KDM6A and KDM6B play an important role in endoderm differentiation from human ESCs. Knockdown of KDM6A or KDM6B impairs endoderm differentiation, which can be rescued by sequential treatment with WNT agonist and antagonist. KDM6A and KDM6B contribute to the activation of WNT3 and DKK1 at different differentiation stages when WNT3 and DKK1 are required for mesendoderm and definitive endoderm differentiation, respectively. Our study not only uncovers an important role of the H3K27me3 demethylases in definitive endoderm differentiation, but also reveals that they achieve this through modulating the WNT signaling pathway.Cell Research advance online publication 21 August 2012; doi:10.1038/cr.2012.119. |
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Authors:
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Wei Jiang; Jinzhao Wang; Yi Zhang |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-8-21 |
Journal Detail:
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Title: Cell research Volume: - ISSN: 1748-7838 ISO Abbreviation: Cell Res. Publication Date: 2012 Aug |
Date Detail:
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Created Date: 2012-8-21 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9425763 Medline TA: Cell Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1] Howard Hughes Medical Institute, Chevy Chase, Maryland 20815-6789, USA [2] Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7295, USA. |
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