Document Detail


Histomorphometric study in children with idiopathic nephrotic syndrome.
MedLine Citation:
PMID:  7563953     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
From 1981 to 1987, renal specimens obtained from 91 children with idiopathic nephrotic syndrome were categorized into 5 histologic subgroups, according to the WHO classification. We analyzed differences in the ratios of the size of the mesangial stalks and attached capillary, including the Bowman's space between the capillary tufts (E), mesangial matrix (Mx), and approximate capillary lumens (E-Mx) to the whole glomerular area of these subgroups using the histomorphometer-IBAS. Focal segmental glomerulosclerosis with diffuse mesangial proliferation (FSGS + DP) had the largest matrix area (Mx/G > 0.5), and smallest capillary lumen size (E/G-Mx/G) (0.3474 +/- 0.0702). On the contrary, FSGS with minimal change (FSGS + MC) had the smallest mesangial matrix (Mx/G), 0.2834 +/- 0.07726, but preserved a larger capillary lumen (0.427 +/- 0.1215). The approximate size of capillary lumens, from the smallest to the largest, was in the following order: FSGS + DP, diffuse mesangial proliferative glomerulonephritis (DPGN), focal segmental proliferative glomerulonephritis (FSPGN), FSGS + MC and minimal change nephrotic syndrome (MCNS). The ratio of the total number of mesangial cells to the area of the whole glomerulus (MCN/G) derived from the light microscopic examination was parallel to the value of Mx/G obtained by the histomorphometric technique. In summary, we introduced the histomorphometric technique using the histomorphometer-IBAS for quantitative measurement of various areas in the glomeruli. The data derived from the system is compatible with those obtained by experienced nephrologists, suggesting that the histomorphometric technique is helpful in histopathology. It is hoped that this new methodology will be used more extensively in the near future.
Authors:
J T Lin; C Y Lin; F Y Huang; P P Lee; C H Chen; H C Hsu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nihon Jinzo Gakkai shi     Volume:  37     ISSN:  0385-2385     ISO Abbreviation:  Nihon Jinzo Gakkai Shi     Publication Date:  1995 Aug 
Date Detail:
Created Date:  1995-11-21     Completed Date:  1995-11-21     Revised Date:  2011-08-01    
Medline Journal Info:
Nlm Unique ID:  7505731     Medline TA:  Nihon Jinzo Gakkai Shi     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  451-5     Citation Subset:  IM    
Affiliation:
Department of Pediatrics, Taipei Medical College Hospital, Taiwan, R.O.C.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Capillaries / ultrastructure
Child
Child, Preschool
Female
Glomerular Mesangium / blood supply,  pathology*
Humans
Kidney Glomerulus / pathology
Male
Nephrotic Syndrome / pathology*

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