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Histomorphology and small intestinal sodium-dependent glucose transporter 1 gene expression in piglets fed phytic acid and phytase-supplemented diets.
MedLine Citation:
PMID:  21454867     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
An experiment was conducted to determine the effect of dietary phytic acid (PA) and phytase supplementation on small intestinal histomorphology and sodium-dependent glucose transporter 1 (SGLT1) gene expression in piglets. Twenty-four piglets with an average initial BW of 7.60 ± 0.73 kg were randomly assigned to 3 experimental diets to give 8 piglets per diet. The diets were a casein-cornstarch-based diet that was supplemented with 0 or 2% PA, or 2% PA (as sodium phytate) plus an Escherichia coli-derived phytase at 500 phytase unit/kg. The basal diet was formulated to meet the 1998 NRC energy, digestible AA, minerals, and vitamins requirements for piglets. After 10 d of feeding, the piglets were killed to determine small intestine histomorphology and small intestinal SGLT1 gene expression. Phytic acid supplementation did not affect villous height (VH) and VH to crypt depth (CD) ratio, but decreased (P < 0.05) CD in the jejunum. Phytase supplementation did not affect VH, CD, and VH to CD ratio. Phytic acid supplementation reduced SGLT1 gene expression in the duodenum, jejunum, and ileum by 1.1-, 5.4-, and 2.4-fold, respectively. Phytase supplementation increased SGLT1 gene expression in jejunum by 2.6-fold, but reduced the expression of the same in the duodenum and ileum by 2.0- and 4.0-fold, respectively. In conclusion, PA reduced the CD in the jejunum and the SGLT1 gene expression in the duodenum, jejunum, and ileum, whereas phytase supplementation increased the expression of the SGLT1 in the jejunum. The reduced SGLT1 gene expression by PA implies that the PA reduces nutrient utilization in pigs partly through reduced expression of the SGLT1 that is involved in glucose and sodium absorption. The increased expression of the SGLT1 in jejunum by phytase supplementation implies that the phytase alleviates the negative effects of PA partly through increased expression of the SGLT1.
Authors:
T A Woyengo; J C Rodriguez-Lecompte; O Adeola; C M Nyachoti
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-3-31
Journal Detail:
Title:  Journal of animal science     Volume:  -     ISSN:  1525-3163     ISO Abbreviation:  -     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-4-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8003002     Medline TA:  J Anim Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Animal Science, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada.
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