Document Detail


Histomorphological evaluation of diabetic and non-diabetic plantar soft tissue.
MedLine Citation:
PMID:  22049867     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
BACKGROUND: Diabetic foot ulceration has a complex and multifactorial etiology and can involve changes in the pathophysiology of the plantar soft tissue. In the current study, histomorphological analyses of diabetic and non-diabetic plantar tissue were performed. It was hypothesized that the diabetic tissue would have thicker skin (epidermis and dermis), less interdigitation between the dermis and epidermis, thicker elastic septa and decreased adipose cell size.
MATERIALS AND METHODS: Two locations of the foot (the heel and the first metatarsal) were examined, both of which have been reported to be locations with a high incidence of ulceration. Stereological methods and quantitative morphological techniques were used to evaluate the skin thickness, interdigitation index, elastic septae thickness and adipocyte cell size.
RESULTS: The diabetic donors had a greater body mass index (BMI) than the non-diabetic donors. The diabetic tissue had significantly thicker elastic septae and dermis. However, no significant difference was observed in the interdigitation index or adipocyte size.
CONCLUSION: These findings demonstrate that morphological changes can be evaluated histologically to give a better understanding of the pathological changes in the plantar soft tissue with diabetes. These evaluations can then be associated with biomechanical changes that occur in diabetes to provide new insight into how microstructural changes can alter macroscopic properties.
CLINICAL RELEVANCE: An understanding of the histomorphological changes in the soft tissue in relationship to the location on the foot could help to explain the biomechanical changes that occur in diabetes and the subsequent increase in susceptibility to breakdown.
Authors:
Yak-Nam Wang; Kara Lee; William R Ledoux
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Foot & ankle international / American Orthopaedic Foot and Ankle Society [and] Swiss Foot and Ankle Society     Volume:  32     ISSN:  1071-1007     ISO Abbreviation:  Foot Ankle Int     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-11-04     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9433869     Medline TA:  Foot Ankle Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  802-10     Citation Subset:  IM    
Affiliation:
VA Puget Sound, RR&D Center of Excellence for Limb Loss Prevention and Prosthetic Engineering, MS 151, 1660 S. Columbian Way, Seattle, WA 98108, USA.
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MeSH Terms
Descriptor/Qualifier:
Grant Support
ID/Acronym/Agency:
1R01 DK75633-03/DK/NIDDK NIH HHS

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