Document Detail

Histometric demonstration of periastrocytic fibronectin loss during herpes simplex virus infection.
MedLine Citation:
PMID:  7938499     Owner:  NLM     Status:  MEDLINE    
Since Herpes simplex virus-type 1 (HSV-1) is liable to induce modifications and/or loss of immunoreactive fibronectin (FN) in cultured cells, our present goal was to determine whether such loss is attributable to FN binding impairment rather than cell detachment secondary to viral cytopathic effect. For this purpose, we resorted herein to an histometric approach for statistical evaluation of FN pattern in the course of HSV-1 infection of astroglial cell monolayers. The length of FN positive fibers was calculated by means of their tracing on a digitizer tablet; in the same field, cell nucleus count was performed. Recorded data allowed the calculation of an index as the ratio of the length of FN positive fibers over cell nucleus number. On comparing the indices between infected and control cultures, matricial FN loss was found in the former at 48 h post-inoculation, accompanied by cell fusion and retraction, though without cell detachment. A significant loss of FN was thus demonstrated as an event prior to severe cytopathic effect induced by viral infection.
C G Castagnino; R F Iacono; M I Berria
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Revista Argentina de microbiología     Volume:  26     ISSN:  0325-7541     ISO Abbreviation:  Rev. Argent. Microbiol.     Publication Date:    1994 Jan-Mar
Date Detail:
Created Date:  1994-10-31     Completed Date:  1994-10-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8002834     Medline TA:  Rev Argent Microbiol     Country:  ARGENTINA    
Other Details:
Languages:  eng     Pagination:  36-41     Citation Subset:  IM    
Departamento de Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
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MeSH Terms
Animals, Newborn
Astrocytes / metabolism,  microbiology*
Cells, Cultured
Cytopathogenic Effect, Viral
Extracellular Matrix / metabolism*
Fibronectins / metabolism*
Nerve Tissue Proteins / metabolism*
Simplexvirus / physiology*
Reg. No./Substance:
0/Fibronectins; 0/Nerve Tissue Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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