Document Detail


Histological studies on early oogenesis in barfin flounder (Verasper moseri).
MedLine Citation:
PMID:  12130808     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
There is much information on oogenesis from the resumption of the first meiotic division to oocyte maturation in many vertebrates; however, there have been very few studies on early oogenesis from oogonial proliferation to the initiation of meiosis. In the present study, we investigated the histological changes during early oogenesis in barfin flounder (Verasper moseri). In fish with a total length (TL) of 50mm (TL 50mm fish), active oogonial proliferation was observed. In TL 60mm fish, oocytes with synaptonemal complexes were observed. Before the initiation of active oogonial proliferation, somatic cells which surrounded a few oogonial germ cells, started to proliferate to form the oogonial cysts that accompanied oogonial proliferation. In TL 70mm fish, however, the cyst structure of the oocyte was gradually broken by the invagination of somatic cells, and finally the oocyte became a single cell surrounded by follicle cells. Upon comparison of nuclear size, DNA-synthesizing germ cells could be divided into two types: small nuclear cells and large nuclear cells. Based on histological observation, we propose that the small nuclear cells were in the mitotic prophase of oogonia and the large nuclear cells were in the meiotic prophase of oocytes, and that the nuclear size increases upon the initiation of meiosis.
Authors:
Toshitsugu Higashino; Takeshi Miura; Chiemi Miura; Kohei Yamauchi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Zoological science     Volume:  19     ISSN:  0289-0003     ISO Abbreviation:  Zool. Sci.     Publication Date:  2002 May 
Date Detail:
Created Date:  2002-07-19     Completed Date:  2002-10-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8702287     Medline TA:  Zoolog Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  557-63     Citation Subset:  IM    
Affiliation:
Division of Marine Biosciences, Graduate School of Fisheries Science, Hokkaido University, Hakodate, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis
Bromodeoxyuridine / metabolism
Cell Division
Female
Flounder / physiology*
Immunohistochemistry
In Situ Nick-End Labeling
Meiosis
Oogenesis*
Ovary / cytology*,  physiology*,  ultrastructure
Chemical
Reg. No./Substance:
59-14-3/Bromodeoxyuridine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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