Document Detail


Histological and genetic studies in patients with bicuspid aortic valve and ascending aorta complications.
MedLine Citation:
PMID:  22194275     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: Aneurysm diameter and growing rate does not represent a definite parameter for operation in bicuspid aortic valve (BAV), ascending aortic aneurysm and normal root patients. Thus, we investigated histological and immunohistochemical aspects of different segments of ascending aorta (precisely, aortic root without dilatation, aneurysmatic tubular portion, dissected ascending aorta) and genetic features of patients with BAV and ascending aorta complication (aneurysm or dissection).
METHODS: Aorta tissue samples of 24 BAV patients were examined. The patients comprised of 18 men and 6 women; the mean age was 54.2 ± 14.3 years. All patients underwent composite aortic root replacement (button Bentall operation). Multiple histological sections were prepared from each aortic specimen. The evaluated features included elastic fibre fragmentation, cystic medial change, smooth muscle cell necrosis, medial fibrosis, and the markers of medial apoptosis and the metalloproteinases. Furthermore, genetic risk factors were also investigated.
RESULTS: The same medial degenerative lesions in tissue samples of different aorta segments (precisely of aortic root without dilatation, and aneurysmatic ascending aorta portion) were observed. More significant associations between single nucleotide polymorphisms (-786T/C endothelial nitric oxide synthase enzyme, D/I angiotensin-converting enzyme, -1562C/T metalloproteinase-9 and -735C/T metalloproteinase-2) and aneurysm risk were detected in BAV patients than in controls.
CONCLUSIONS: Based on our histological and genetic data, we underline that a surgical approach in patients with BAV, ascending aortic aneurysm and normal root, should consider not only the diameter of the aneurysmatic aortic portion but also the histological features of the whole ascending aorta and the genetic risk profile.
Authors:
Calogera Pisano; Emiliano Maresi; Carmela Rita Balistreri; Giuseppina Candore; Daniele Merlo; Khalil Fattouch; Giuseppe Bianco; Giovanni Ruvolo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-12-22
Journal Detail:
Title:  Interactive cardiovascular and thoracic surgery     Volume:  14     ISSN:  1569-9285     ISO Abbreviation:  Interact Cardiovasc Thorac Surg     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-02-22     Completed Date:  2012-07-16     Revised Date:  2013-04-15    
Medline Journal Info:
Nlm Unique ID:  101158399     Medline TA:  Interact Cardiovasc Thorac Surg     Country:  England    
Other Details:
Languages:  eng     Pagination:  300-6     Citation Subset:  IM    
Affiliation:
Unit of Cardiac Surgery, Department of Surgery and Oncology, University of Palermo, Palermo, Italy. bacalipi@libero.it
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MeSH Terms
Descriptor/Qualifier:
Aneurysm, Dissecting / diagnosis,  genetics*,  metabolism
Aorta, Thoracic / pathology*,  ultrasonography
Aortic Aneurysm, Thoracic / diagnosis,  genetics*,  metabolism
Aortic Valve / abnormalities*,  pathology,  ultrasonography
Apoptosis
Biological Markers / metabolism
DNA / genetics*
Echocardiography, Doppler, Color
Echocardiography, Transesophageal
Female
Follow-Up Studies
Genetic Predisposition to Disease
Genotype
Heart Valve Diseases / congenital,  diagnosis,  genetics*
Humans
Immunohistochemistry
In Situ Nick-End Labeling
Male
Middle Aged
Polymerase Chain Reaction
Polymorphism, Single Nucleotide*
Retrospective Studies
Risk Factors
Chemical
Reg. No./Substance:
0/Biological Markers; 9007-49-2/DNA
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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