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Histological characterization and immunohistochemical localization of ki-67 and VEGF factors in bitches' corpus luteum at cyclic and early and late gestational diestrous.
MedLine Citation:
PMID:  23279467     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The luteal phase on pregnant and non-pregnant bitches is characteristic of this species and resembles significantly with respect to the growth pattern and luteal regression. Histological and immunostaining studies of the corpus luteum (CL) may help to elucidate differences between the CL of pregnant and non-pregnant bitches. The purpose of this study was to characterize histologically and localize by immunohistochemistry the cell proliferation (Ki-67) and vascular endothelial growth (VEGF) factors in the CL of pregnant and non-pregnant bitches. Eighteen bitches were analysed and distributed into three groups: In group I (gestational diestrous), seven bitches were subjected to two inseminations at 4 and 6 days after the pre-ovulatory LH surge and ovariohysterectomized (OSH) at 8-21 days after the first insemination. In group II (cyclic diestrous; control), 6 (Ki-67) or 8 (VEGF) bitches that were determined as non-pregnant were OSH at 12-25 days of the pre-ovulatory LH surge. In group III (late pregnancy), three bitches had their ovary removed during caesarean section at 62-64 days after the pre-ovulatory LH surge. Portions of the ovarian cortex containing CLs were cut and stored for histological and immunohistochemical analysis. Histological evaluation of the ovarian cortex showed a marked similarity in the morphological pattern among the CLs in all three groups. The morphology and expression pattern of VEGF and Ki-67 factors in CLs of cyclic and gestational diestrous bitches were similar but significantly lower than that of late pregnant bitches (p < 0.05).
Authors:
Aa Ribeiro; I Martin; Aap Derussi; Rb Ferioli; R Laufer-Amorim; Md Lopes
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Reproduction in domestic animals = Zuchthygiene     Volume:  47 Suppl 6     ISSN:  1439-0531     ISO Abbreviation:  Reprod. Domest. Anim.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2013-01-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9015668     Medline TA:  Reprod Domest Anim     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  61-5     Citation Subset:  IM    
Copyright Information:
© 2012 Blackwell Verlag GmbH.
Affiliation:
Department of Animal Reproduction and Veterinary Radiology, College of Veterinary Medicine and Animal Science, UNESP, Botucatu, Sao Paulo, Brazil.
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