Document Detail


Histological changes in the skin of Rana pipiens produced by either KCl loading or fasting.
MedLine Citation:
PMID:  7700887     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present study was performed to determine if either potassium loading or fasting results in histological changes in the skin. Rana pipiens were loaded with KCl and skin biopsies obtained (Group I). These biopsies were compared with biopsies from NaCl loaded frogs (Group II). In blind studies of microscopic sections, 13 of 17 biopsies of a mixture of I and II were correctly diagnosed by one observer and similarly, 14 of 17 of Group I and II were correctly diagnosed by a second observer (P = 0.0245 and 0.0063, respectively). The characteristics used to distinguish skins from KCl treated frogs versus controls treatment included: (i) an abundance of large euchromatin cells on or near the surface; (ii) changes in the basal cell layer with elongation and rotation of the nuclei; (iii) lighter cells in the spinosal layers; and (iv) sometimes the skin became thicker. The water-soluble nondialyzable material of the frog skin was extracted, and we found that it increased by 4.4 times following KCl loading (P < 0.05). However, the protein fraction was not increased by loading the frog for 3 days with NaHCO3. We conclude that potassium loading results in characteristic histological changes in the skin and that this is probably related to the ability of the skin to excrete potassium. In addition, a comparison of biopsies of skin from fed frogs with samples from frogs fasted for 40 to 49 days showed a change in the thickness of the skin. Skins of fed frogs averaged 57.0 +/- 1.4 mu thick compared with fasted, 39.9 +/- 2.7 mu (P < 0.001).
Authors:
J C Vanatta; I H al-Hashimi; L W Frazier
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)     Volume:  208     ISSN:  0037-9727     ISO Abbreviation:  Proc. Soc. Exp. Biol. Med.     Publication Date:  1995 Apr 
Date Detail:
Created Date:  1995-04-28     Completed Date:  1995-04-28     Revised Date:  2007-11-02    
Medline Journal Info:
Nlm Unique ID:  7505892     Medline TA:  Proc Soc Exp Biol Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  385-90     Citation Subset:  IM    
Affiliation:
Department of Physiology, University of Texas Southwestern Medical School, Dallas 75235.
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MeSH Terms
Descriptor/Qualifier:
Animals
Epithelial Cells
Epithelium / drug effects
Fasting*
Female
Male
Potassium Chloride / pharmacology*
Rana pipiens
Reference Values
Skin / cytology*,  drug effects
Sodium Chloride / pharmacology*
Chemical
Reg. No./Substance:
7447-40-7/Potassium Chloride; 7647-14-5/Sodium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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