Document Detail


Histologic evaluation of demineralized freeze-dried bone allografts in barrier membrane covered periodontal fenestration wounds and ectopic sites in dogs.
MedLine Citation:
PMID:  15191589     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND/AIM: The aim of this study was to investigate healing responses to demineralized freeze-dried bone powder allografts in standardized periodontal fenestration defects, compared with subcutaneous wounds in a dog model. METHODS: Circular periodontal fenestration defects were created buccally at all four canines in 14 mongrel dogs. Each site received one of the following underneath a barrier membrane: (a) ethylene oxide (EO)-sterilized demineralized freeze-dried bone allografts (DFDBA), (b) heat-treated DFDBA, (c) non-sterilized DFDBA and (d) ungrafted control. Twelve of the 14 dogs had three subcutaneous chest wall pouches created and one of the three DFDBA materials placed in each. The animals were necropsied at 4 weeks. Histologic sections were prepared through the center of the fenestration sites in an apico-coronal direction. Quantitative analysis using computer-assisted imaging technique was performed. Subcutaneous implants were evaluated histologically and quantified for associated inflammatory cell infiltrate. RESULTS: Fenestration defects healed by partial osseous fill and cementum regeneration with formation of a periodontal ligament. The graft particles generally appeared isolated from the site of osteogenesis and covered by cementum-like substance. Graft particles incorporated into newly formed bone at a distance from the root surface was the exception. No statistically significant differences in new bone formation were observed between treatment groups within animals, but significant inter-animal variation was found (p<0.01). Quantities of retained graft particles were limited, and without cellular resorption. A bone augmentation effect was associated with the barrier in the majority of sites. No bone formation was evident at the subcutaneous sites where graft particles displayed distinctly modified surface zones and multinucleated giant cell resorption. Significantly more inflammatory infiltrate was associated with EO-sterilized grafts compared with heat-treated grafts (p=0.05). CONCLUSION: Implantation of DFDBA neither enhanced osseous healing in periodontal fenestration defects, nor resulted in ectopic bone induction. DFDBA particles implanted in either periodontal fenestration or subcutaneous wounds evoked distinctly different healing responses.
Authors:
Morten Klepp; James E Hinrichs; Ted Eastlund; Erwin M Schaffer
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of clinical periodontology     Volume:  31     ISSN:  0303-6979     ISO Abbreviation:  J. Clin. Periodontol.     Publication Date:  2004 Jul 
Date Detail:
Created Date:  2004-06-11     Completed Date:  2004-07-26     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0425123     Medline TA:  J Clin Periodontol     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  534-44     Citation Subset:  D; IM    
Affiliation:
morten.klepp@stavanger.online.no
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MeSH Terms
Descriptor/Qualifier:
Alveolar Bone Loss / surgery*
Analysis of Variance
Animals
Bone Demineralization Technique
Bone Matrix / transplantation*
Bone Regeneration*
Bone Transplantation / methods*
Dental Cementum / physiology
Dogs
Ethylene Oxide
Female
Freeze Drying
Guided Tissue Regeneration, Periodontal / methods*
Hot Temperature
Male
Membranes, Artificial
Statistics, Nonparametric
Sterilization / methods
Transplantation, Heterotopic*
Wound Healing
Chemical
Reg. No./Substance:
0/Membranes, Artificial; 75-21-8/Ethylene Oxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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