Document Detail


Histochemistry: live and in color.
MedLine Citation:
PMID:  21339179     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Histochemistry-chemistry in the context of biological tissue-is an invaluable set of techniques used to visualize biological structures. This field lies at the interface of organic chemistry, biochemistry, and biology. Integration of these disciplines over the past century has permitted the imaging of cells and tissues using microscopy. Today, by exploiting the unique chemical environments within cells, heterologous expression techniques, and enzymatic activity, histochemical methods can be used to visualize structures in living matter. This review focuses on the labeling techniques and organic fluorophores used in live cells.
Authors:
Luke D Lavis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  59     ISSN:  1551-5044     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-02-22     Completed Date:  2011-04-11     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  139-45     Citation Subset:  IM    
Affiliation:
Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147, USA. lavisl@janelia.hhmi.org
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Survival
Color
Fluorescent Dyes / chemistry,  metabolism
Genetic Engineering
Histocytochemistry / methods*
Humans
Ligases / metabolism
Grant Support
ID/Acronym/Agency:
//Howard Hughes Medical Institute
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; EC 6.-/Ligases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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