Document Detail


Histochemistry defines a proteoglycan-rich layer in bovine flexor tendon subjected to bending.
MedLine Citation:
PMID:  15788872     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mid-substance fibrocartilage develops in bovine deep flexor tendon at the point where the tendon wraps under sesamoid bones of the foot and receives transverse compressive loading during locomotion. Fibrocartilage extends several millimeters into the tendon at this location and the proteoglycan-rich tissue stains intensely with Alcian blue. Using histochemical techniques we demonstrate the presence of aggrecan, type VI collagen, and hyaluronic acid in the extracellular matrix of this region of tendon. Biglycan staining was localized to the cells, however. Adjacent to the fibrocartilage, at the outer curvature of the tendon as it bends, the tissue resembles typical tensile tendon with dense bundles of linearly arranged collagen. Longitudinal sections revealed discrete layers of Alcian blue-stained material between the collagen bundles. We demonstrate that these layers of loose matrix also contain aggrecan, type VI collagen, and hyaluronic acid. However, the dense collagen bundles of this region are negative for these components. Transverse sections of tendon in the area adjacent to fibrocartilage show a distinct Alcian blue-stained structure surrounding vascular elements at the point where several fiber bundles come together. This is concluded to be the same structure as the Alcian blue-stained layers seen in longitudinal sections. These observations suggest that proteoglycan-rich matrices in tendon subjected to mechanical loading other than pure tension may serve multiple roles. Such matrices can not only provide compressive stiffness and separate and lubricate collagen bundles that move relative to each other, but may also protect the integrity of vasculature in tendon subjected to bending and shear.
Authors:
K G Vogel; J A Peters
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of musculoskeletal & neuronal interactions     Volume:  5     ISSN:  1108-7161     ISO Abbreviation:  J Musculoskelet Neuronal Interact     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-03-24     Completed Date:  2005-08-18     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  101084496     Medline TA:  J Musculoskelet Neuronal Interact     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  64-9     Citation Subset:  IM    
Affiliation:
Department of Biology, The University of New Mexico, Albuquerque, NM 87131, USA. kgvogel@unm.edu
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MeSH Terms
Descriptor/Qualifier:
Aggrecans
Animals
Blood Vessels / physiology
Cattle
Collagen Type VI / metabolism
Elasticity
Extracellular Matrix / chemistry*,  metabolism*
Extracellular Matrix Proteins / metabolism
Hyaluronic Acid / metabolism
Lectins, C-Type
Proteoglycans / analysis,  metabolism*
Stress, Mechanical
Tendons / chemistry*,  cytology,  metabolism*
Weight-Bearing / physiology
Grant Support
ID/Acronym/Agency:
AR36110/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
0/Aggrecans; 0/Collagen Type VI; 0/Extracellular Matrix Proteins; 0/Lectins, C-Type; 0/Proteoglycans; 123939-84-4/biglycan; 9004-61-9/Hyaluronic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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