Document Detail

Histochemical and topo-optical investigations on tissue-isolated and in vitro amyloid fibrils.
MedLine Citation:
PMID:  16194873     Owner:  NLM     Status:  MEDLINE    
An improved microscopic slide preparation for staining of tissue-isolated and in vitro amyloid fibrils is described in detail. Specimens were prepared by slow drying of an amyloid fibril suspension on aminoalkyl-silanised glass slides underneath a coverglass. The purpose of this method is to obtain microscopically visible fibers. Polarization microscopy was performed on unstained fibers in different embedding media, on fibers stained with Congo red, toluidine blue, 1,9-dimethylmethylene blue, anisotropic PAS reaction and Sudan black. Results were identical in 8 tissue-isolated amyloid fibril preparations. The refractive index of amyloid was about 1.5, as expected for protein fibrils. Congo red-stained fibrils showed yellow or blue-green polarization colors, while staining with toluidine blue or 1,9-dimethylmethylene blue induced green or orange polarization colors. The latter dyes stained the fibrils even in buffers with low pH or high ionic strength, pointing to strong acidic groups like sulphates in amyloid. The anisotropic PAS reaction for selective demonstration of periodate reactive carbohydrates in tissue-isolated amyloid fibrils was positive. Thus, linearly ordered acidic groups and carbohydrate residues could be detected for the first time in tissue-isolated amyloid fibrils using an improved technique for microscopic slide preparation on one side and topo-optical reactions on the other.
Thomas R Appel; Susann Richter; Reinhold P Linke; Josef Makovitzky
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Amyloid : the international journal of experimental and clinical investigation : the official journal of the International Society of Amyloidosis     Volume:  12     ISSN:  1350-6129     ISO Abbreviation:  Amyloid     Publication Date:  2005 Sep 
Date Detail:
Created Date:  2005-09-30     Completed Date:  2008-03-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9433802     Medline TA:  Amyloid     Country:  United States    
Other Details:
Languages:  eng     Pagination:  174-83     Citation Subset:  IM    
Leibniz Institute of Molecular Biotechnology, Jena, Germany.
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MeSH Terms
Amyloid / chemistry*,  metabolism*
Azo Compounds
Carbohydrate Metabolism
Carbohydrates / chemistry
Congo Red
Insulin / chemistry,  metabolism
Methylene Blue / analogs & derivatives
Myoglobin / chemistry,  metabolism
Organ Specificity
Staining and Labeling*
Tolonium Chloride
Reg. No./Substance:
0/Amyloid; 0/Azo Compounds; 0/Carbohydrates; 0/Myoglobin; 11061-68-0/Insulin; 23481-50-7/1,9-dimethylmethylene blue; 4197-25-5/Sudan Black B; 573-58-0/Congo Red; 61-73-4/Methylene Blue; 92-31-9/Tolonium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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