Document Detail


Histochemical studies of the differentiation of microglial cells in the cerebral hemispheres of chick embryos and chicks.
MedLine Citation:
PMID:  2820907     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Using histochemical procedures to reveal the presence of nucleoside diphosphatase (NDPase), thiamine pyrophosphatase (TPPase) and acid phosphatase (AcPase), we investigated the appearance, distribution and ultrastructure of amoeboid and microglial cells in the cerebral hemispheres of chick embryos and young chicks, in order to elucidate the relationship between these two cell populations. On day 6 of incubation, a few round cells exhibiting NDPase, TPPase and AcPase activity were first detected in the thin mantle layer of the cerebral hemisphere. In the corpus striatum, these round cells increased rapidly in abundance until day 13 of incubation, after which their numbers gradually decreased, so that, on day 19 of incubation, they had entirely disappeared. Between day 10 and day 17 or 18 of incubation, round cells were located mainly in the zone of the mantle layer closest to the lumen. On day 10 of incubation, NDPase-, TPPase- and AcPase-positive cells that had a few short cytoplasmic processes (poorly ramified cells) were detected in the intermediate and basal zones of mantle layer. They increased in abundance until day 17 or 18 of incubation and thereafter rapidly decreased in number. Round and poorly ramified cells exhibited NDPase activity on their plasma membranes and in their cytoplasmic vacuoles, with TPPase and AcPase activity being localized within their vacuoles. On day 19 of incubation, NDPase- and TPPase-positive cells with long, well-ramified cytoplasmic processes (well-ramified cells) were observed in the corpus striatum, these being mainly localized in the basal zone. After hatching, these cells increased rapidly in abundance and were distributed throughout the corpus striatum.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
E Fujimoto; A Miki; H Mizoguti
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Histochemistry     Volume:  87     ISSN:  0301-5564     ISO Abbreviation:  Histochemistry     Publication Date:  1987  
Date Detail:
Created Date:  1987-11-17     Completed Date:  1987-11-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0411300     Medline TA:  Histochemistry     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  209-16     Citation Subset:  IM    
Affiliation:
Department of Anatomy, Kobe University School of Medicine, Japan.
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MeSH Terms
Descriptor/Qualifier:
Acid Anhydride Hydrolases*
Acid Phosphatase / metabolism
Animals
Brain / cytology*,  embryology
Cell Differentiation
Chick Embryo
Chickens
Corpus Striatum / cytology
Histocytochemistry
Lysosomes / enzymology
Neuroglia / cytology*,  enzymology
Phosphoric Monoester Hydrolases / metabolism
Thiamine Pyrophosphatase / metabolism
Chemical
Reg. No./Substance:
EC 3.1.3.-/Phosphoric Monoester Hydrolases; EC 3.1.3.2/Acid Phosphatase; EC 3.6.-/Acid Anhydride Hydrolases; EC 3.6.1.-/Thiamine Pyrophosphatase; EC 3.6.1.6/nucleoside-diphosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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