| Histochemical fluorescence of tissue and brain monoamines: results in 18 minutes using the sucrose-phosphate-glyoxylic acid (SPG) method. | |
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MedLine Citation:
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PMID: 11370236 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A rapid and sensitive modification of the glyoxylic acid method for the histofluorescent visualization of catecholamines and serotonin in mammalian brain, and other tissues, from cryostat sections is described. The procedure uses a phosphate-sucrose buffer solution combined with 1% glyoxylic acid. Tissues are reacted for 3 s at room temperature. Catecholaminergic cell bodies, pre-terminal, and terminal varicosities, well-localized and brightly fluorescent, are seen throughout cortical and subcortical structures. Serotoninergic cells show a weak fluorescence in rat and mouse brain but a strong fluorescence in dog and monkey brain. |
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Authors:
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J C de la Torre; J W Surgeon |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Neuroscience Volume: 1 ISSN: 0306-4522 ISO Abbreviation: Neuroscience Publication Date: 1976 Dec |
Date Detail:
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Created Date: 2001-05-23 Completed Date: 2001-07-12 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7605074 Medline TA: Neuroscience Country: United States |
Other Details:
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Languages: eng Pagination: 451-3 Citation Subset: IM |
Affiliation:
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University of Chicago School of Medicine, Division of Neurosurgery, Brain Research Institute, 950 E. 59th Street, Chicago, IL, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biogenic Monoamines / analysis* Brain Chemistry / physiology* Buffers Dogs Glyoxylates / diagnostic use* Histocytochemistry / methods* Macaca mulatta Mice Microscopy, Fluorescence / methods* Neurons / chemistry*, cytology Phosphates / diagnostic use Potassium Compounds / diagnostic use Rats Sucrose / diagnostic use Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Biogenic Monoamines; 0/Buffers; 0/Glyoxylates; 0/Phosphates; 0/Potassium Compounds; 16068-46-5/potassium phosphate; 298-12-4/glyoxylic acid; 57-50-1/Sucrose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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