| Histochemical discrimination of fibers in regenerating rat infraorbital nerve. | |
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MedLine Citation:
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PMID: 1375308 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In rat dorsal root ganglia, histochemical staining of carbonic anhydrase (CA) and cholinesterase (CE) yields a reciprocal pattern of activity: Sensory processes are CA positive and CE negative, whereas motor processes are CA negative and CE positive. In rat infraorbital nerve (a sensory peripheral nerve), we saw extensive CA staining of nearly 100% of the myelinated axons. Although CE reactivity in myelinated axons was extremely rare, we did observe CE staining of unmyelinated autonomic fibers. Four weeks after transection of infraorbital nerves, CA-stained longitudinal sections of the proximal stump demonstrated 3 distinct morphological zones. A fraction of the viable axons retained CA activity to within 2 mm of the distal extent of the stump, and the stain is capable of resolving growth sprouts being regenerated from these fibers. Staining of unmyelinated autonomic fibers in serial sections shows that CE activity was not retained as far distally as is the CA sensory staining. |
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Authors:
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R A Wilke; D A Riley; J R Sanger |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Microsurgery Volume: 13 ISSN: 0738-1085 ISO Abbreviation: Microsurgery Publication Date: 1992 |
Date Detail:
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Created Date: 1992-06-25 Completed Date: 1992-06-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8309230 Medline TA: Microsurgery Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 39-44 Citation Subset: IM; S |
Affiliation:
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Department of Cellular Biology and Anatomy, Medical College of Wisconsin, Milwaukee. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Axons / ultrastructure Carbonic Anhydrases Cholinesterases Ganglia, Spinal / ultrastructure Histocytochemistry Male Maxillary Nerve / ultrastructure Motor Neurons / ultrastructure* Nerve Degeneration Nerve Fibers / ultrastructure* Nerve Fibers, Myelinated / ultrastructure Nerve Regeneration* / physiology Neurons, Afferent / ultrastructure* Orbit / innervation* Rats Rats, Inbred Strains Spinal Nerve Roots / ultrastructure Staining and Labeling |
| Chemical | |
Reg. No./Substance:
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EC 3.1.1.8/Cholinesterases; EC 4.2.1.1/Carbonic Anhydrases |
| Investigator | |
Investigator/Affiliation:
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D A Riley / Med Coll WI, Milwaukee |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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