Document Detail


Histochemical detection of lipid droplets in cultured cells.
MedLine Citation:
PMID:  23027019     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Cells store excess lipid as esters in the form of triglycerides and cholesterol esters. Most lipid esters are compartmentalized in globular structures called lipid droplets. Here we describe several methods of detecting lipid droplets by fluorescence microscopy. Lipid droplets can be visualized either by staining the lipid ester core using fluorescent dyes or by labeling lipid droplet-specific proteins using antibodies. The intracellular distribution of lipid droplets can be analyzed without much difficulty by these methods, but care must be taken to avoid certain pitfalls.
Authors:
Michitaka Suzuki; Yuki Shinohara; Toyoshi Fujimoto
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  931     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-10-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  483-91     Citation Subset:  IM    
Affiliation:
Department of Anatomy and Molecular Cell Biology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
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