| Histochemical analysis of glycoconjugates in gelatinous membranes of the gerbil's inner ear. | |
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MedLine Citation:
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PMID: 1757294 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The gelatinous membranes of the gerbil inner ear were analyzed histochemically for glycoconjugates with a battery of twenty horseradish peroxidase-conjugated lectins. Glycoconjugates with mannose (Man) and/or glucose (Glc), galactose (Gal), fucose (Fuc), N-acetylglucosamine (GlcNAc), N-acetylgalactosamine (GalNAc) and N-acetylneuraminic acid (NeuAc) were detected in the tectorial and otolithic membranes and cupula. Differences in lectin reactivity were observed between tectorial and vestibular membranes and also among zones and between the medial and lateral regions of the middle zone of the tectorial membrane. The distribution of staining differed markedly for several lectins that bind specifically to GalNAc or to GlcNAc but vary in affinity for oligosaccharides containing these sugars in different sequences or linkages. The findings suggest presence of the terminal disaccharides GalNAc alpha 1,3Gal in tectorial membrane and Gal beta 1,3GalNAc in vestibular membranes. Lectin binding profiles provided evidence that the limbal zone's fibrous and attachment layers contain mainly O-glycosidically linked oligosaccharides whereas the middle zone's medial fibrous layer contains both O- and N-linked chains. The remaining regions of the tectorial membrane contain mainly N-linked oligosaccharides with bisected biantennary type chains predominating. Additionally, the marginal band and the middle zone's basal layer contain abundant N-linked oligosaccharides with a triantennary structure. |
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Authors:
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S Sugiyama; S S Spicer; P D Munyer; B A Schulte |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Hearing research Volume: 55 ISSN: 0378-5955 ISO Abbreviation: Hear. Res. Publication Date: 1991 Oct |
Date Detail:
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Created Date: 1992-02-06 Completed Date: 1992-02-06 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7900445 Medline TA: Hear Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 263-72 Citation Subset: IM; S |
Affiliation:
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Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston 29425. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbohydrate Metabolism Carbohydrate Sequence Carbohydrates / chemistry Ear, Inner / metabolism* Gerbillinae Glycoconjugates / chemistry, metabolism* Histocytochemistry Lectins / metabolism Molecular Sequence Data Otolithic Membrane / metabolism Tectorial Membrane / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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DC00422/DC/NIDCD NIH HHS; DC00713/DC/NIDCD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Carbohydrates; 0/Glycoconjugates; 0/Lectins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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