Document Detail

Histochemical analysis of glycoconjugate secretion in the alimentary canal of Anguilla anguilla L.
MedLine Citation:
PMID:  15707657     Owner:  NLM     Status:  MEDLINE    
Conventional histochemical methods as well as lectin-binding techniques were used to study glycoconjugates that are present in the alimentary canal of the European eel (Anguilla anguilla). Specimens from pharynx, oesophagus, stomach and intestine were collected from adult ("silver eel" stage) females. Alcian Blue pH 2.5/PAS and High Iron Diamine/Alcian Blue pH 2.5 reactions were performed to stain neutral and acidic glycoconjugates. In addition, lectin histochemistry was applied to identify acidic glycoconjugates containing O-acylated sialic acids. Finally, the presence of sugar residues in the oligosaccharide side chains of glycoconjugates were investigated by using biotinylated lectins. Acidic and neutral glycoconjugates were found to be secreted throughout the alimentary canal, the acidic glycoconjugates appeared to be either sialylated or sulphated. Sialylated glycoconjugates were identified to contain sialic acid substituted at carbon in position 7 (C7). Sulphated glycoconjugates were particularly abundant in the distal intestine and were not present in the secretory products of the gastric mucosa, which contained a variety of sugar residues (D-N-acetyl-galactosamine, beta-D-galactose, alpha-D-mannose, alpha-L-fucose, D-N-acetyl-glucosamine). Lectin binding was observed in mucous cells of pharynx, oesophagus and intestine, and particularly some monosaccharides (D-N-acetyl-galactosamine and beta-D-galactose) were abundantly present.
Cinzia Domeneghini; Silvana Arrighi; Giuseppe Radaelli; Giampaolo Bosi; A Veggetti
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-01-11
Journal Detail:
Title:  Acta histochemica     Volume:  106     ISSN:  0065-1281     ISO Abbreviation:  Acta Histochem.     Publication Date:  2005  
Date Detail:
Created Date:  2005-02-14     Completed Date:  2005-08-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370320     Medline TA:  Acta Histochem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  477-87     Citation Subset:  IM    
Department of Veterinary Sciences and Technologies for Food Safety, Faculty of Veterinary Medicine, University of Milan, via Trentacoste n.2, Milan 20134, Italy.
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MeSH Terms
Digestive System / cytology,  secretion*
Eels / anatomy & histology,  metabolism*
Glycoconjugates / secretion*
Reg. No./Substance:

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