| Histidine decarboxylase: isolation and molecular characteristics. | |
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MedLine Citation:
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PMID: 6504233 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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High levels of histidine decarboxylase activity were measured in rat basophilic leukemia cells grown in ascitic form in 4 week old WKY/N rats. The potent inhibition of this enzyme by brocresine and alpha-methylhistidine but not by alpha-methyl DOPA identified it as a specific histidine decarboxylase. Gel filtration and polyacrylamide gel electrophoresis revealed a molecular weight of 125,000 for the native enzyme, similar to that of fetal rat liver histidine decarboxylase. Using rat basophilic leukemia cells as starting material, histidine decarboxylase was purified extensively in a seven step procedure. Electrophoresis under denaturing conditions revealed that histidine decarboxylase is a dimeric protein consisting of two identical subunits with a molecular weight of 62,000. The results indicate that rat basophilic leukemia cells provide a new and rich source for the purification of histidine decarboxylase. |
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Authors:
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R Grzanna |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Neurochemical research Volume: 9 ISSN: 0364-3190 ISO Abbreviation: Neurochem. Res. Publication Date: 1984 Jul |
Date Detail:
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Created Date: 1985-01-03 Completed Date: 1985-01-03 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7613461 Medline TA: Neurochem Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 993-1009 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Basophils* Carboxy-Lyases / isolation & purification* Chromatography, Gel Chromatography, Ion Exchange Electrophoresis, Polyacrylamide Gel Histidine Decarboxylase / isolation & purification* Isoelectric Focusing Leukemia / enzymology* Molecular Weight Neoplasm Transplantation Rats Rats, Inbred WKY |
| Grant Support | |
ID/Acronym/Agency:
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NS-15199/NS/NINDS NIH HHS; NS-16654/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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EC 4.1.1.-/Carboxy-Lyases; EC 4.1.1.22/Histidine Decarboxylase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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