Document Detail

Histidine decarboxylase in human basophilic leukemia (KU-812-F) cells. Characterization and induction by phorbol myristate acetate.
MedLine Citation:
PMID:  2117926     Owner:  NLM     Status:  MEDLINE    
The human leukemic cell line KU-812-F is known to differentiate into mature basophil-like cells under serum-free culture conditions. In the present study, the activity of histidine decarboxylase (HDC), a histamine-forming enzyme, in KU-812-F cells was found to be high, ranging from 10 to 57 pmol/min/mg protein. The great variation in HDC activity appeared to be due to different percentages and degrees of maturity of basophil-like cells during differentiation of this cell line. The enzyme was inhibited by alpha-fluoromethylhistidine but not by carbidopa, was unable to form dopamine from L-3,4-dihydroxyphenylalanine, and had a Km value for histidine of 0.27 mM, indicating that it was HDC and not aromatic amino acid decarboxylase. The HDC activity increased 1.8-fold when the cells were stimulated by phorbol myristate acetate, which is known to activate protein kinase C, and this increase was blocked by staurosporine, a potent inhibitor of protein kinase C.
R Mamune-Sato; Y Tanno; K Maeyama; Y Miura; T Takishima; K Kishi; T Fukuda; T Watanabe
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical pharmacology     Volume:  40     ISSN:  0006-2952     ISO Abbreviation:  Biochem. Pharmacol.     Publication Date:  1990 Sep 
Date Detail:
Created Date:  1990-09-27     Completed Date:  1990-09-27     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0101032     Medline TA:  Biochem Pharmacol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1125-9     Citation Subset:  IM    
First Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
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MeSH Terms
Alkaloids / pharmacology
Calcimycin / pharmacology
Carboxy-Lyases / metabolism*
Cycloheximide / pharmacology
Enzyme Induction / drug effects
Histidine Decarboxylase / metabolism*
Leukemia, Basophilic, Acute / enzymology*,  metabolism
Protein Kinase C / antagonists & inhibitors*
Tetradecanoylphorbol Acetate / pharmacology*
Tumor Cells, Cultured / drug effects
Reg. No./Substance:
0/Alkaloids; 16561-29-8/Tetradecanoylphorbol Acetate; 52665-69-7/Calcimycin; 62996-74-1/Staurosporine; 66-81-9/Cycloheximide; EC Kinase C; EC 4.1.1.-/Carboxy-Lyases; EC Decarboxylase

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