| Histidine decarboxylase expression as a new sensitive and specific marker for small cell lung carcinoma. | |
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MedLine Citation:
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PMID: 12527716 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Histidine decarboxylase is one of the enzymes of the amine precursor uptake and decarboxylation system and is known to be distributed in mast cells and enterochromaffin-like cells. With the hypothesis that histidine decarboxylase expression is a marker for neuroendocrine differentiation, we studied the immunoreactivity of histidine decarboxylase in neuroendocrine cells and tumors of the thyroid gland, adrenal medulla, lung, and gastrointestinal tract. Formalin-fixed paraffin sections were subjected to immunohistochemistry using anti-histidine decarboxylase antibody, and the sensitivity and specificity were compared with those of conventional neuroendocrine markers (CD56, chromogranin A, synaptophysin, and neuron-specific enolase). Enterochromaffin or enterochromaffin-like cells, adrenal chromaffin cells, and thyroid C-cells were positive for histidine decarboxylase, and related tumors (carcinoid tumor, pheochromocytoma, medullary carcinoma) showed a high percentage of positive staining. Furthermore, we used the antibody to distinguish small cell lung carcinoma from non-neuroendocrine lung carcinoma and also to detect neuroendocrine differentiation in large-cell neuroendocrine carcinoma and gastrointestinal small-cell carcinoma. The anti-histidine decarboxylase antibody stained most small cell lung carcinoma (18 of 23, sensitivity 0.78), and was rarely reactive with non-neuroendocrine lung tumors (2 of 44; specificity, 0.95). These values were close to those obtained from CD56 staining (sensitivity/specificity, 0.87/0.98). Histidine decarboxylase was also positive for 6 of 12 large cell neuroendocrine carcinomas and 4 of 7 gastrointestinal small cell carcinomas. In conclusion, we demonstrated that histidine decarboxylase is useful to distinguish between small cell lung carcinoma and non-neuroendocrine carcinoma and to demonstrate neuroendocrine differentiation. |
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Authors:
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Yasumasa Matsuki; Akihide Tanimoto; Tetsuo Hamada; Yasuyuki Sasaguri |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc Volume: 16 ISSN: 0893-3952 ISO Abbreviation: Mod. Pathol. Publication Date: 2003 Jan |
Date Detail:
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Created Date: 2003-01-15 Completed Date: 2003-07-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8806605 Medline TA: Mod Pathol Country: United States |
Other Details:
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Languages: eng Pagination: 72-8 Citation Subset: IM |
Affiliation:
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Department of Pathology and Cell Biology, School of Medicine, University of Occupational and Environmental Health, Yahatanishi, Kitakyushu, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, CD56
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analysis Carcinoma, Small Cell / chemistry, enzymology*, pathology Chromogranin A Chromogranins / analysis Histidine Decarboxylase / metabolism* Humans Immunoenzyme Techniques Lung Neoplasms / chemistry, enzymology*, pathology Phosphopyruvate Hydratase / analysis Sensitivity and Specificity Tumor Markers, Biological / analysis |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD56; 0/Chromogranin A; 0/Chromogranins; 0/Tumor Markers, Biological; EC 4.1.1.22/Histidine Decarboxylase; EC 4.2.1.11/Phosphopyruvate Hydratase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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