Document Detail

Histidase expression is regulated by dietary protein at the pretranslational level in rat liver.
MedLine Citation:
PMID:  9566987     Owner:  NLM     Status:  MEDLINE    
The effect of dietary protein on the expression of histidase (Hal) was investigated to understand the mechanism of induction of histidase by a high protein diet. In this study, we examined the following: 1) the effect of 0, 6, 18, 35 and 50% casein diets on hepatic and epidermal Hal activity, amount of the enzyme and Hal-mRNA; 2) the effect of a high histidine diet (1.25%) on Hal expression; 3) the response of Hal expression in rats fed a 10% casein diet and injected with glucagon (0.6 mg /(100 g body wt.d); and 4) the half-lives of the enzyme and Hal-mRNA in rats fed an 80% casein diet for 7 d followed by a protein-free diet. Hal activity increased as the protein content in the diet increased (r = 0.986, P < 0.001) and was associated with a significant increase in Vmax without a change in Km. The dietary regulation was liver specific because skin Hal was unresponsive. Increments in hepatic Hal activity were accompanied by concomitant significant increases in the amount of histidase and its mRNA. The response was more pronounced in rats fed diets containing >18% casein. Rats fed a 12% casein diet containing 1.25% histidine did not have different Hal activity and mRNA levels compared with rats fed a 12% casein diet, indicating that Hal expression is not modified by its substrate. Injection of glucagon into rats fed the 10% casein diet increased Hal activity threefold and Hal- mRNA expression fivefold compared with uninjected rats fed the same diet. The apparent half-life of hepatic histidase in protein-depleted rats previously fed an 80% casein diet was 2.8 d, whereas the half-life of Hal-mRNA was 17 h. In summary, these data support the hypothesis that Hal expression is regulated by dietary protein at the pretranslational level in rat liver, and that glucagon is one of the hormones involved in the induction of Hal.
N Torres; L Martínez; G Alemán; H Bourges; A R Tovar
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of nutrition     Volume:  128     ISSN:  0022-3166     ISO Abbreviation:  J. Nutr.     Publication Date:  1998 May 
Date Detail:
Created Date:  1998-06-08     Completed Date:  1998-06-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0404243     Medline TA:  J Nutr     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  818-24     Citation Subset:  IM    
Departamento de Fisiología de la Nutrición, Instituto Nacional de la Nutrición "Salvador Zubiran," México, D. F. 14000, México.
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MeSH Terms
Base Sequence
Blotting, Northern
Circadian Rhythm
DNA Primers / chemistry
Dietary Proteins / administration & dosage*
Gene Expression Regulation, Enzymologic / genetics*
Histidine / administration & dosage
Histidine Ammonia-Lyase / genetics*,  metabolism*
Liver / chemistry,  enzymology*,  metabolism
Polymerase Chain Reaction
Protein Biosynthesis
Random Allocation
Rats, Wistar
Skin / chemistry,  enzymology
Time Factors
Weight Gain
Reg. No./Substance:
0/DNA Primers; 0/Dietary Proteins; 71-00-1/Histidine; EC Ammonia-Lyase

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