Document Detail


Histamine and the stomach: chemical histamine assays.
MedLine Citation:
PMID:  1828307     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Histamine assays in gastroduodenal tissues and body fluids are not an absolute objective of scientific interest but are related to the role of histamine in health and disease. Hence, the reliability of histamine assays has to be assessed in relation to this aim. Sensitivity and specificity of the chemical histamine assays are similar in tissues and body fluids. The modern developments in a fluorometric-fluoroenzymatic assay guarantee the highest sensitivity and specificity, especially by tests that monitor specificity in each single run of histamine determinations. Precision and accuracy of histamine measurement were especially investigated for the fluorometric assay. They included tests on the coefficient of variation over the whole concentration range, long-term precision with double-sample standard control charts, comparison of several methods for histamine assay including bioassay, and long-term accuracy with the use of Cusum charts. Finally, appropriate sample preparation, sample-taking, relevant body fluids and tissues, and the right time for sample-taking were evaluated in extended methodologic studies. Histamine assays are not just methods for a normal routine laboratory. Extended knowledge about histamine release and metabolism will be necessary to analyse data in this particular field with reasonable validity.
Authors:
W Lorenz; K Thon; H Stöltzing; E Neugebauer; R Lindlar; J Sattler; D Weber
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Publication Detail:
Type:  Journal Article; Meta-Analysis; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Scandinavian journal of gastroenterology. Supplement     Volume:  180     ISSN:  0085-5928     ISO Abbreviation:  Scand. J. Gastroenterol. Suppl.     Publication Date:  1991  
Date Detail:
Created Date:  1991-07-11     Completed Date:  1991-07-11     Revised Date:  2008-02-13    
Medline Journal Info:
Nlm Unique ID:  0437034     Medline TA:  Scand J Gastroenterol Suppl     Country:  NORWAY    
Other Details:
Languages:  eng     Pagination:  9-25     Citation Subset:  IM    
Affiliation:
Institute of Theoretical Surgery, University of Marburg/Lahn, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Assay
Chromatography, High Pressure Liquid / standards
Decision Trees
Fluorometry / standards
Gas Chromatography-Mass Spectrometry / standards
Gastric Mucosa / chemistry*
Histamine / analysis*
Humans
Intestinal Mucosa / chemistry*
Meta-Analysis as Topic
Peptic Ulcer / metabolism*
Radioimmunoassay / standards
Sensitivity and Specificity
Chemical
Reg. No./Substance:
51-45-6/Histamine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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