| Histamine stimulates ion transport by dog pancreatic duct epithelial cells through H1 receptors. | |
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MedLine Citation:
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PMID: 9655687 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Histamine affects pancreatic secretion, but its direct action on ion transport by pancreatic duct epithelial cells (PDEC) has not been defined. We now characterize the secretory effects of histamine on cultured, well-differentiated, and nontransformed dog PDEC. Histamine stimulated, in a concentration-dependent manner (1-100 microM), a cellular 125I- efflux that was inhibited by 500 microM 5-nitro-2-(3-phenylpropylamino)benzoic acid, 2.5 mM diphenylamine-2-carboxylate, and 500 microM DIDS and thus mediated through Ca2+-activated Cl- channels. Histamine-stimulated 125I- efflux was 1) inhibited by 100 microM diphenhydramine, an H1 receptor antagonist, 2) resistant to 1 mM cimetidine, an H2 receptor antagonist, 3) not reproduced by 1 mM dimaprit, an H2 agonist, and 4) inhibited by 50 microM 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid-AM, a Ca2+ chelator, suggesting that it was mediated through H1 receptors acting via increased cytosolic Ca2+. Histamine also stimulated a 86Rb+ efflux that was sensitive to 100 nM charybdotoxin and thus mediated through Ca2+-activated K+ channels. When PDEC monolayers were studied in Ussing chambers, a short-circuit current of 21.7 +/- 3.1 microA/cm2 was stimulated by 100 microM histamine. This effect was inhibited by diphenhydramine but not cimetidine, was not reproduced with dimaprit, and was observed only after serosal addition of histamine, suggesting that it was mediated by basolateral H1 receptors on PDEC. In conclusion, histamine, acting through basolateral H1 receptors, activates both Ca2+-activated Cl- and K+ channels; in this manner, it may regulate PDEC secretion in normal or inflamed pancreas. |
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Authors:
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T D Nguyen; C N Okolo; M W Moody |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: The American journal of physiology Volume: 275 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1998 Jul |
Date Detail:
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Created Date: 1998-08-25 Completed Date: 1998-08-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: G76-84 Citation Subset: IM |
Affiliation:
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Department of Medicine, University of Washington, and Veterans Affairs Puget Sound Health Care System, Seattle, Washington 98108, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
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pharmacology Animals Anthranilic Acids / pharmacology Calcium / metabolism* Calcium Channel Blockers / pharmacology Cells, Cultured Chelating Agents / pharmacology Chlorides / metabolism* Cimetidine / pharmacology Dimaprit / pharmacology Diphenhydramine / pharmacology Dogs Egtazic Acid / analogs & derivatives, pharmacology Epithelial Cells / drug effects, physiology* Histamine / pharmacology* Iodides / metabolism Iodine Radioisotopes Kinetics Nitrobenzoates / pharmacology Pancreatic Ducts / physiology* Receptors, Histamine H1 / drug effects, physiology* |
| Chemical | |
Reg. No./Substance:
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0/Anthranilic Acids; 0/Calcium Channel Blockers; 0/Chelating Agents; 0/Chlorides; 0/Iodides; 0/Iodine Radioisotopes; 0/Nitrobenzoates; 0/Receptors, Histamine H1; 139890-68-9/1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester; 158913-21-4/4-nitro-2-(3-phenylpropylamino)benzoate; 51-45-6/Histamine; 51481-61-9/Cimetidine; 53005-05-3/4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; 58-73-1/Diphenhydramine; 65119-89-3/Dimaprit; 67-42-5/Egtazic Acid; 7440-70-2/Calcium; 91-40-7/fenamic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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