Document Detail

Histamine promotes the expression of receptors TLR2 and TLR4 and amplifies sensitivity to lipopolysaccharide and lipoteichoic acid treatment in human gingival fibroblasts.
MedLine Citation:
PMID:  21418040     Owner:  NLM     Status:  MEDLINE    
The vasoactive hydrophilic amine histamine is the most important molecule released by mast cells. However, histamine's role in activating intracellular responses in HGFs (human gingival fibroblasts) has not been evaluated to date. In the present study, we investigated the effect of histamine and of Gram-negative [LPS (lipopolysaccharide)] and Gram-positive [LTA (lipoteichoic acid)] bacterial components on the modulation of the inflammatory response of HGFs. We incubated HGFs with histamine to determine whether this hydrophilic amine regulates overexpression of TLR2 (Toll-like receptor 2) and TLR4, which recognize LTA and LPS respectively. Our experiments demonstrated that histamine increases transcription and translation of TLR2 and TLR4. Incubation with LTA or LPS in the presence of histamine markedly increased expression of COX2 (cyclo-oxygenase 2) and synthesis of prostaglandin E2. These results suggest that histamine plays an important role in modulating the innate immune response, and likewise, that LTA and LPS regulate the adaptive immune response. The present study provides information about the regulation and expression of molecules that promote chronic inflammatory processes leading to the emergence of periodontitis and the consequent loss of the dental organ.
Gloria Gutiérrez-Venegas; Sandra Cruz-Arrieta; Mónica Villeda-Navarro; José Antonio Méndez-Mejía
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biology international     Volume:  35     ISSN:  1095-8355     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-09-21     Completed Date:  2012-01-11     Revised Date:  2012-08-02    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  1009-17     Citation Subset:  IM    
Laboratorio de Bioqumica, Divisin de Estudios de Posgrado e Investigacin, Universidad Nacional Autnoma de Mxico, DF, Mexico.
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MeSH Terms
Cyclooxygenase 2 / metabolism
Dinoprostone / metabolism
Fibroblasts / drug effects*,  metabolism
Gene Expression Regulation* / drug effects
Gingiva / cytology
Histamine / pharmacology*
Lipopolysaccharides / pharmacology*
Teichoic Acids / pharmacology*
Toll-Like Receptor 2 / genetics,  metabolism
Toll-Like Receptor 4 / genetics,  metabolism*
Reg. No./Substance:
0/Lipopolysaccharides; 0/Teichoic Acids; 0/Toll-Like Receptor 2; 0/Toll-Like Receptor 4; 363-24-6/Dinoprostone; 51-45-6/Histamine; 56411-57-5/lipoteichoic acid; EC 2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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