Document Detail

Histamine and methacholine aerosol bronchial challenge in awake guinea pigs.
MedLine Citation:
PMID:  2184218     Owner:  NLM     Status:  MEDLINE    
Cardiopulmonary responses to histamine and methacholine aerosol challenge were examined and compared in unanesthetized spontaneously breathing guinea pigs. All animals had surgically implanted intrapleural catheters and some had arterial or right heart catheters as well. Animals were placed in plethysmographs and exposed to ascending doses of aerosolized agonists. The provocative dose, i.e., the dose that caused a clear bronchospastic response, was defined as the concentration that increased intrapleural pressure to at least 20 cm H2O. Results showed that bronchospasm was characterized further by decreases in dynamic lung compliance and arterial PO2 and an increase in airway resistance. The accumulation of trapped gas in the lung, after challenge, measured in the excised collapsed lung correlated with the increase in intrapleural pressure and with the fall in lung compliance. Male guinea pigs between 3- and 15-weeks of age did not vary in sensitivity to histamine. Methacholine and histamine were equipotent and produced similar responses. Tachyphylaxis was not demonstrated for either compound. In addition, the provocative dose for the drugs remained stable on a day-to-day basis. These results offer insight into the nature of the bronchospastic response in guinea pigs and provide new data on response to methacholine.
M J Wiester; J S Tepper; M F Weber; M G Ménache
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  The Journal of pharmacology and experimental therapeutics     Volume:  253     ISSN:  0022-3565     ISO Abbreviation:  J. Pharmacol. Exp. Ther.     Publication Date:  1990 Apr 
Date Detail:
Created Date:  1990-05-30     Completed Date:  1990-05-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376362     Medline TA:  J Pharmacol Exp Ther     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  27-33     Citation Subset:  IM    
Pulmonary Toxicology Branch, U.S. Environmental Protection Agency, Research Triangle Park, North Carolina.
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MeSH Terms
Age Factors
Airway Resistance / drug effects
Bronchi / drug effects*
Bronchial Provocation Tests
Guinea Pigs
Histamine / pharmacology*
Lung Compliance / drug effects
Methacholine Chloride
Methacholine Compounds / pharmacology*
Oxygen / blood
Time Factors
Reg. No./Substance:
0/Aerosols; 0/Methacholine Compounds; 51-45-6/Histamine; 62-51-1/Methacholine Chloride; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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