| Histamine inhibits cell proliferation and modulates the expression of Bcl-2 family proteins via the H2 receptor in human pancreatic cancer cells. | |
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MedLine Citation:
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PMID: 17201167 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this study, the mechanisms involved in the inhibitory effect of histamine (HA) on PANC-1 cell proliferation were investigated. The action of HA on cell growth was evaluated by determining the cell doubling time from experimental growth curves and analysing the cell cycle using a flow cytometer. The expression of proteins related to cell death and proliferation (PCNA, p53, c-Fos and Bcl-2 family proteins) was studied using Western blot, immunocytochemistry and flow cytometric analysis. The results indicated that HA produced an accumulation of PANC-1 cells in GO/Gl-phase and increased the doubling time via H2HA (H2R) stimulation. Expression of p53, c-Fos and Bcl-2 were not modulated by HA. However, HA decreased PCNA and Bax expression, while it increased the Bcl-x level. In summary, the antiproliferative effect exerted by HA was associated with a G0/G1-phase arrest and a modulation of the Bcl-2 family proteins. |
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Authors:
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Graciela Cricco; Gabriela Martín; Vanina Medina; Mariel Núñez; Nora Mohamad; Máximo Croci; Ernesto Crescenti; Rosa Bergoc; Elena Rivera |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Anticancer research Volume: 26 ISSN: 0250-7005 ISO Abbreviation: Anticancer Res. Publication Date: 2006 Nov-Dec |
Date Detail:
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Created Date: 2007-01-04 Completed Date: 2007-01-25 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8102988 Medline TA: Anticancer Res Country: Greece |
Other Details:
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Languages: eng Pagination: 4443-50 Citation Subset: IM |
Affiliation:
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Radioisotopes Laboratory, School of Pharmacy and Biochemistry, University of Buenos Aires, Argentina. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Death
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drug effects Cell Line, Tumor Cell Proliferation / drug effects* Flow Cytometry Histamine / pharmacology* Humans Immunohistochemistry Proto-Oncogene Proteins c-bcl-2 / physiology* Receptors, Histamine H2 / physiology* |
| Chemical | |
Reg. No./Substance:
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0/Proto-Oncogene Proteins c-bcl-2; 0/Receptors, Histamine H2; 51-45-6/Histamine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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