Document Detail

Histamine inhibits cell proliferation and modulates the expression of Bcl-2 family proteins via the H2 receptor in human pancreatic cancer cells.
MedLine Citation:
PMID:  17201167     Owner:  NLM     Status:  MEDLINE    
In this study, the mechanisms involved in the inhibitory effect of histamine (HA) on PANC-1 cell proliferation were investigated. The action of HA on cell growth was evaluated by determining the cell doubling time from experimental growth curves and analysing the cell cycle using a flow cytometer. The expression of proteins related to cell death and proliferation (PCNA, p53, c-Fos and Bcl-2 family proteins) was studied using Western blot, immunocytochemistry and flow cytometric analysis. The results indicated that HA produced an accumulation of PANC-1 cells in GO/Gl-phase and increased the doubling time via H2HA (H2R) stimulation. Expression of p53, c-Fos and Bcl-2 were not modulated by HA. However, HA decreased PCNA and Bax expression, while it increased the Bcl-x level. In summary, the antiproliferative effect exerted by HA was associated with a G0/G1-phase arrest and a modulation of the Bcl-2 family proteins.
Graciela Cricco; Gabriela Martín; Vanina Medina; Mariel Núñez; Nora Mohamad; Máximo Croci; Ernesto Crescenti; Rosa Bergoc; Elena Rivera
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anticancer research     Volume:  26     ISSN:  0250-7005     ISO Abbreviation:  Anticancer Res.     Publication Date:    2006 Nov-Dec
Date Detail:
Created Date:  2007-01-04     Completed Date:  2007-01-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  4443-50     Citation Subset:  IM    
Radioisotopes Laboratory, School of Pharmacy and Biochemistry, University of Buenos Aires, Argentina.
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MeSH Terms
Cell Death / drug effects
Cell Line, Tumor
Cell Proliferation / drug effects*
Flow Cytometry
Histamine / pharmacology*
Proto-Oncogene Proteins c-bcl-2 / physiology*
Receptors, Histamine H2 / physiology*
Reg. No./Substance:
0/Proto-Oncogene Proteins c-bcl-2; 0/Receptors, Histamine H2; 51-45-6/Histamine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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