Document Detail


Hispolon Induces Apoptosis and Cell Cycle Arrest of Human Hepatocellular Carcinoma Hep3B Cells by Modulating ERK Phosphorylation.
MedLine Citation:
PMID:  21630638     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Hispolon is an active phenolic compound of Phellinus igniarius, a mushroom that has recently been shown to have antioxidant, anti-inflammatory, and anticancer activities. In this study, we investigated the antiproliferative effect of hispolon on human hepatocellular carcinoma Hep3B cells by using the MTT assay, DNA fragmentation, DAPI (40, 6-diamidino-2-phenylindole dihydrochloride) staining and flow cytometric analyses. Hispolon inhibited cellular growth of Hep3B cells in a time-dependent and dose-dependent manner, through the induction of cell cycle arrest at S phase measured using flow cytometric analysis and apoptotic cell death, as demonstrated by DNA laddering. Hispolon-induced S-phase arrest was associated with a marked decrease in the protein expression of cyclins A, and E and cyclin-dependent kinases (CDKs) 2, with concomitant induction of p21waf1/Cip1 and p27Kip1. Exposure of Hep3B cells to Hispolon resulted in apoptosis as evidenced by caspase activation, PARP cleavage, and DNA fragmentation. Hispolon treatment also activated JNK, p38 MAPK and ERK expression. Inhibitors of ERK (PB98095), but not those of JNK (SP600125) and p38 MAPK (SB203580), suppressed hispolon-induced S-phase arrest and apoptosis in Hep3B cells. These findings establish a mechanistic link between MAPK pathway, and hispolon-induced cell cycle arrest and apoptosis in Hep3B cells.
Authors:
Guan-Jhong Huang; Jeng-Shyan Deng; Shyh-Shyun Huang; Miao-Lin Hu
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-6-1
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  -     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-6-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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